内毒素致兔急性肺损伤时内源性肺表面活性物质的改变

Alterations of the Endogenous Surfactant Syatem in Acute Lung Injury lnduced by Escherichia Coli Lipopolysaccharide in A Rabbit Model

探索脓毒症引起的急性肺损伤（ＡＬＩ）时内源性肺表面活性物质（ＰＳ）主要成分的变化。方法制作兔脓毒性ＡＬＩ模型，进行支气管肺泡灌洗液（ＢＡＬＦ）磷脂、功能亚型聚集体及活性的分析，Ｗｅｓｔｅｒｎｄｏｔｂｌｏｔ法和Ｎｏｒｔｈｅｒｎｂｌｏｔ法测肺表面活性物质结合蛋白（ＳＰＡ）含量及ｍＲＮＡ含量的变化。结果肺损伤后与正常组比，总磷脂（ＴＰＬ）（７．８±１．１）ｖｓ（１２．７±１．７）ｍｇ／ｋｇ，饱和磷脂占ＴＰＬ百分比（ＤＳＰＣ／ＴＰＬ）（２６．６±５．６）％ｖｓ（３５．７±４．８）％，ＳＰ－Ａ蛋白（８０．８±１８．２）ｖｓ（１７３．７±１５．５）μｇ／ｋｇ，ＳＰ－ＡｍＲＮＡ５０．３±８．９ｖｓ８２．１±１２．９，含量均显著减少。总蛋白（ＴＰ）（１００．９±７．３）ｖｓ（４９．３±２．７）ｍｇ／ｋｇ，小聚积体（ＳＡ）与大聚集体（ＬＡ）的比例（ＳＡ／ＬＡ）１．１１±０．１５ｖｓ０．５４±０．１，最小表面张力（γｍｉｎ）（２０．２±０．８）ｖｓ（８．３±５．９）ｍＮ／ｍ，均有显著的异常升高。结论脓毒性ＡＬＩ时内源性ＰＳ系统主要成分发生了质量和功能的异常损害。

Purpose To determine alternations of endogenous pulmonary surfactant (PS) in most common septic acute lung injury(ALI). Methods We established an acute lung injury model in rabbits induced by Escherichia coli lipopolysaccharide (LPS),analysed the phosphorus,subtypes and activity of broncho alveol lavage fluid (BALF),exa mined the SP A protion in BALF by Western dot blot,SP A mRNA in lung tissue by Northern blot . Results The results demonstrated that the total phosphorus(TPL) was (7.8±1.1) vs (12.7±1.7)mg/kg,disaturated phodphatidylcholine (DSPC/TPL) (26.6±5.6)% vs (35.7±4.8)%,SP A protion (80.8±18.2) vs ( 173.7 ±15.5)μg/kg SPA mRNA 50.3±8.9 vs 82.1±12.9 all decreased, total protion (TP) (100.9±7.3) vs (49.3±2.7)mg/kg,small aggregate (SA) and large aggregate (LA) ratio (SA/LA) 1.11±0.15 vs 0.54±0.1 and minimam tention of BALF (γ min )(20.2±0.8) vs (8.3±5.9)mN/m increased singnificantly. Conclusions Endogenous surfactant system was significantly injured in amount,subtypes and activities in septic ALI,it may be the internal causes why the course of ALI is difficult to control.