斑点免疫结合试验直接检测末梢血甲胎蛋白的研究和应用

THE STUDY AND APPLICATION OF DOT IMMUNOBINDING ASSAY FOR DIRECT DETECTION OF ALPHA FETOPROTElN IN FULL BLOOD

目的：探讨建立一种方便使用、检测人末梢血甲胎蛋白（AFP）的方法。方法：用斑点免疫结合试验（DIBA）直接检测人末梢血（全血）AFP。结果：DIBA检测的条件在37℃或室温，孵育时间为10min×2时，敏感度达25μg／L。用此法和放射免疫法同时检测住院肝癌病人90例的末梢血和血清AFP，结果符合率为96.67％，以AFP50μg／L判为阴性，两结果的阳性符合率达100％。并用此法检测普查的正常人群1200例，检出末梢血AFP浓度为50～100ug／L有3例，与血清DIBA和RIA检测结果相符。结论：DIBA试剂盒检测人末梢血（全血）AFP，具有特异性强、敏感度高，与常规检测法有较好的符合性，而且具有简便、省料、快速、可直接检测末梢血（全血）等优点。

To establish a simple method for detecting human alph fetoprotein (AFP) in full blood. Method: Establlshing and using a dot immunobindin assay (DIBA) for direct detection of AFP in full blood. ResuIts: The sensitivity of DIBA for the AFP was 25 μg/L when the incubation was conducted in 37℃or in room temperature and the incubation time was 10 minutes×2. When AFP concentrations of 90 full blood samples from patlents with cancer were detected by the method and at the same time by radioimmunoassay, it was showed that the coincidence rate of the two methods was up to 96. 67% and the coincidence rate in positive was 100% (when of AFP concentration of less than 50μg/L was negative). The concentrations of three in 1 200 full blood samples were 50 t0 100 μg/L and the other less than 50 μg/L when DIBA was adopted. The re-sults were the same as th0se produced by RIA method. Conclusion: In detection of AFP in full blood, DIBA shows its advantage of specificity, sensitivity and simplicity, and it is fast and economical, thus can be used wide-ly in hospltal labs and general survey for liver cancer.