超声与双水相体系耦合提取竹叶总黄酮研究

Extraction of Total Flavonoids from Bamboo Leaf Based on Propyl-alcohol Ammonium Sulfate Aqueous Two-phase System Coupling with Ultrasonic

[目的]探讨超声提取法和双水相分离技术相结合提取竹叶黄酮的新方法。[方法]利用超声与丙醇-硫酸铵双水相体系耦合对竹叶总黄酮进行提取和分离,并研究硫酸铵和丙醇含量、超声时间和液固比对黄酮提取率的影响。[结果]利用超声与丙醇-硫酸铵双水相体系耦合对竹叶总黄酮进行提取分离的最佳提取条件为:丙醇体积分数60%;(NH4)2SO4用量为0.35g/ml,超声提取25min、液固比为30∶1,在此条件下,竹叶总黄酮提取率为2.03%,提取物中总黄酮纯度为31.3%,明显高于常规超声法。[结论]超声与丙醇-硫酸铵双水相体系耦合对竹叶总黄酮提取的方法简便,值得推广应用。

[Objective] To investigate a propyl-alcohol ammonium sulfate aqueous two-phase system coupling with ultrasonic for extraction of total flavonoids from bamboo leaf. [Method] The propyl-alcohol ammonium sulfate aqueous two-phase system coupling with ultrasonic was used to extract and separate the total flavonoids from bamboo leaf. The effects of the ammonium sulfate and propanol content,ultrasonic time and fluid-solid ratio on the extraction yield of flavonoids were also studied. [Result] The optimum extraction conditions are as follows：propanol volume fraction 60%; （NH4）2SO4 0.35 g/ml,ultrasonic 25 min,solution to materials ratio 30：1. The extraction rates of total flavonoids was 2.03% in the optimum extraction conditions,and the purity of total flavonoids in extractives was 31.3%%,much higher than that of ultrasonic extraction. [Conclusion] The propyl-alcohol ammonium sulfate aqueous two-phase system coupling with ultrasonic used for extraction of total flavonoids from bamboo is simple and has good prospects.