不同pH值条件下铜绿微囊藻与附生假单胞菌磷代谢研究

Study on Phosphorus Metabolism of Microcystis aeruginosa and Adhesive Pseudomonas sp. under different pH Value

[目的]探讨pH值对铜绿微囊藻的生长代谢及其与附生假单胞菌磷代谢关系的影响。[方法]通过不同pH值条件下(8～10)铜绿微囊藻的吸磷试验和不同pH值(7.0～9.0)对铜绿微囊藻与假单胞菌磷代谢的影响试验,测定了短时间内铜绿微囊藻的吸磷情况,以及较长时间内微囊藻的生长曲线、水中磷浓度的变化和微囊藻中总磷含量的变化。[结果]短时间内,pH值对铜绿微囊藻的吸磷能力有影响,随着pH值的升高,吸磷能力增强。在较长一段时间内,pH值越高,藻的生长速率越快,生长情况越好。铜绿微囊藻有自我调节pH的能力,能很好地利用假单胞菌释放到水体中的磷。在藻、菌、水的系统中,菌中的磷起缓释磷源的作用,尽管浓度很低,但有利于藻的生长。[结论]pH值是影响藻-菌-水系统中磷元素循环的因素。

[Objective] The study aimed to discuss the effects of pH value on the growth metabolism of Microcystis aeruginosa and its relation to the metabolism of adhesive Pseudomonas sp.. [Method] In the experiment on absorbing phosphorus by M. aeruginosa under different pH at 8-10 and in the experiment on the effects of different pH at 7.0-9.0 on the metabolism of M. aeruginosa and adhesive Pseudomonas sp.,the phosphorus uptake of M. aeruginosa in a short time was measured and the growth curve,the change of phosphorus concn. in the water and the change of total phosphorus content in M. aeruginosa in longer time were also measured. [Result] In the short time,pH value had some effects on the ability of attracting the phosphorus by M. aeruginosa,and the ability of attracting the phosphorus by M. aeruginosa was enhanced along with the rising of pH value. During a longer time,the high pH value could accelerate the growth of M. aeruginosa,which grew better in the system consisted of M. aeruginosa,adhesive Pseudomonas sp. M. aeruginosa had the ability of self regulation of pH value,and could use the phosphorus in the water released from its adhesive Pseudomonas sp.. The phosphorus in fungus could play the action of delaying the release of phosphorus source in the system of alga,fungus and water. Though the phosphorus concn. was lower,it was be propitious to the growth of the alga. [Conclusion] pH value was the factor that affected the circle of the phosphorus element in the system of alga,fungus and water.