摘要
[目的]探讨Vc对黑色素癌细胞A375的生长抑制作用。[方法]利用MTT法分析不同剂量组的Vc对A375增殖的影响,并通过荧光染色和荧光显微镜观察A375细胞的形态学变化,用流式细胞术检测Vc对各组A375的细胞周期变化。[结果]流式方法测定的结果显示,Vc对黑色素癌细胞A375的增殖具有抑制作用;Vc能以浓度依赖和时间依赖方式阻滞黑色素癌细胞A375细胞于G0/G1期,抑制其生长并诱导凋亡。[结论]Vc对黑色素癌细胞的生长有明显抑制作用。
[Objective] The aim was to analyze the inhibition effects of Vc on melanoma A375 cells. [Method] The effects of different doses Vc on A375 cells proliferation was tested by MTT method,and the morphological changes of A375 cells were observed by fluorescence staining and fluorescence microscopy,and the A375 cell cycle was checked by flow cytometry. [Result] Vc could inhibit the growth of melanoma A375 cells,and the suppressive effect was increased with treat concentration and treat time,the cell cycle was visibly blocked at G0/G1 phase. [Conclusion] The proliferation of melanoma A375 cells could be inhibited obviously by Vc.
出处
《安徽农业科学》
CAS
北大核心
2010年第23期12748-12749,12784,共3页
Journal of Anhui Agricultural Sciences
基金
宁夏自然基金(NZ0905)
宁夏大学自然基金项目(ZR200726)