衰老标记蛋白-30——一种新的肝细胞癌相关抗原

SENESCENCE MARKER PROTEIN 30 A NEW ASSOCIATED ANTIGEN BY HEPATOCELLULAR CARCINOMA

目的：寻找人肝细胞癌（ＨＣＣ）特异抗原。方法：应用重组克隆表达抗原的血清学鉴定技术（ＳＥＲＥＸ），用病人自身血清从广西肝细胞癌ｃＤＮＡ文库中筛选出编码ＨＣＣ肿瘤抗原的基因克隆，用肝癌患者及其他人血清检验ＨＣＣ肿瘤抗原对肝癌的特异性，测定抗原ＤＮＡ序列并与基因库核对寻找其同源结构。结果：其中一种ＨＣＣ肿瘤抗原与衰老标记蛋白－３０（ＳＭＰ－３０）是同源结构。相应抗体主要只见于ＨＣＣ病人，在２０例其它５种恶性肿瘤和４例肝硬化病人未查到相应抗体，２０例慢性肝炎病人血清和２０例正常人血清均只见１例呈阳性反应。结论：衰老标记蛋白－３０为一种新发现的ＨＣＣ相关抗原，对ＨＣＣ有诊断学意义。

Objective:To investigate specific antigens of hepatocelluar carcinoma (HCC) Methods:Serological identification of antigens by recombinant expression cloning (SEREX) technique was used for screening of specific antigens of hepatocelluar carcinoma cDNA library of hepatocellular carcinoma tissues from Guangxi.These antigens reacted to the sera of other persons,and analysis of these clonic gene sequences was conducted Results:One of these antigens--HOM HCC 22 5,was homologous with human mRNA for senescence marker protein 30 (SMP 30) Serologic test demonstrated that 3/5 HCC had positive reaction to HOM HCC 22 5 Twenty sera from different kinds of neoplasm and four from hepatocirrhosis were all negative Conclusion:The results of sera analysis suggest that HOM HCC 22 5/SMP 30 is a new HCC associated antigen and will be of great value to diagnosis of HCC