碱性成纤维细胞生长因子促进小型猪创面修复的剂量与效应关系研究

STUDY OF DOSAGE-EFFECT RELATIONSHIP BETWEEN THE WOUND-REPAIRING OF PIGS AND BASIC FIBROBLAST GROWTH FACTOR

目的：研究碱性成纤维细胞生长因子（ｂＦＧＦ）促进小型猪创面修复和对表皮分离细胞培养的量效关系。方法：利用小型猪创伤模型，分别按３个不同浓度的ｂＦＧＦ组（５００Ｕ／ｍｌ、１０００Ｕ／ｍｌ、１５００Ｕ／ｍｌ）处理创面，并用磺胺嘧啶银（ＳＤ－Ａｇ）及生理盐水做对照，观察创面愈合情况；同时取相应部位的表皮细胞进行培养，ＭＴＴ法观察ｂＦＧＦ对猪表皮细胞体外生长的影响。结果：提示１０００Ｕ／ｍｌ及１５００Ｕ／ｍｌ浓度组促创伤修复作用比较明显，体外细胞培养１０～５０μｇ／Ｌ细胞增殖作用最明显。结论：ｂＦＧＦ对促进小型猪创面修复和对表皮分离细胞培养有一定的量效关系。

Objective:To study the dosage-effect relations of bFGF in promoting wound healing of pigs and culturing dissociated epidermic cells.Methods:The wound models of pigs were treated separately by 3 different concentrations of bFGF,SD-Ag and saline. Dissociated epidermic cells of corresponding sites were cultured and the effects of bFGF were observed using MTT method in vitro.Results:The wound healing effect of the groups of 1 000U/ml and 1 500U/ml bFGF were obvious andin vitro,the growth of epidermic cells were most excellent in 10～50μg/L bFGF.Conclusion:bFGF have obvious dosage-effects in promoting wound healing of pigs and culturing dissociated epidermic cell.