女性肺癌的血管生成和血管内皮生长因子的表达研究

Angiogenesis and the expression of vascular endothelial growth factor \=in female patients with nonsmall cell lung cancer

目的研究女性非小细胞性肺癌（ＮＳＣＬＣ）中微血管密度（ＭＶＤ）和血管内皮生长因子（ＶＥＧＦ）的表达，并分析其与病理分期、组织类型间的关系。方法应用免疫组织化学技术，检测了４０例女性ＮＳＣＬＣ外科手术标本中ＭＶＤ和ＶＥＧＦ表达。其中鳞癌７例，腺癌２７例，混合型鳞腺癌６例。外科病理分期为Ⅰ期１３例，Ⅱ～Ⅲ期２７例。结果（１）３７例女性ＮＳＣＬＣ中ＶＥＧＦ阳性率平均为７８．４％，其中Ⅰ期病例的阳性率为５８．３％，Ⅱ～Ⅲ期病例的阳性率为８４．０％。ＶＥＧＦ表达在不同病期间无统计学显著差异。（２）４０例女性ＮＳＣＬＣ中ＭＶＤ平均为６．７±３．２，最高达１５。其中Ⅱ～Ⅲ期病例的ＭＶＤ（７．５±２．８）明显高于Ⅰ期病例（５．３±２．３），具有统计学显著差异（Ｐ＜０．０１）。（３）ＭＶＤ与ＶＥＧＦ的表达相关，ＭＶＤ≥６的女性ＮＳＣＬＣ之ＶＥＧＦ阳性率（９０．５％）高于ＭＶＤ＜６的女性ＮＳＣＬＣ（６２．５％），两者差异显著（Ｐ＜０．０２５）。结论在女性非小细胞肺癌中，癌细胞高表达ＶＥＧＦ者血管生成活跃，并且ＭＶＤ增高与病期进展相关。

objective To investigate the microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) in female patients with nonsmall cell lung cancer (NSCLC) and analyze their relationship with stage of disease as well as pathological types. MethodsBZThe expression of VEGF and MVD were examined immunohistochemically in 40 female patients with NSCLC,including 7 cases of squamous cell carcinoma,27 of adenocarcinoma and 6 of adenosquamous carcinoma.Surgical pathology indicated 13 cases in stage and 27 in stage to . ResultsBZ(1)In 37 cases of female NSCLC tested,the incidence of positive staining for VEGF was 78.4%,among which 58.3% in stage and 84.0% in stage .There was no statistically significant difference in the VEGF expression between these two groups.(2)A mean MVD of 6.73. 2 was counted in 40 female NSCLC,with the maximum MVD being 15.There was a significant difference in MVD between stage (7.52.8) and stage (5.32.3) (P<0.01).(3)A significant correlation between MVD and the vascular endothelial growth factorfemaleVEGF expression was established,VEGF expression rate in MVD6 group (90.5%) was significantly higher than that in MVD<6 group (62.5%).This difference in VEGF expression was statistically significant (P<0.025). T5HZConclusion These results indicated that in female NSCLC,the active angiogenesis existed in the tumors with higher expression of VEGF.MVD,as an index of angiogenesis,showed a good correlation with the stage of disease.\;