诺氟沙星葡萄糖注射液细菌内毒素检测的探讨

The discussion on the endotoxin inspection method of norflovacin and gloucose injection

目的：建立诺氟沙星葡萄糖注射液的细菌内毒素检查法。方法：根据中国药典１９９５版二部收载的细菌内毒素检查法的要求进行实验。结果：将诺氟沙星葡萄糖注射液经４倍稀释后可排除干扰因素，用标示灵敏度为０．１２５Ｅｕ·ｍｌ－１的鲎试剂检测细菌内毒素是有效的。结论：可以用鲎试验法代替家兔法作为医院日常检测诺氟沙星葡萄糖注射液的热原检查。

OBJECTIVE :To establish the endotoxin inspection method of norflovacin and gloucose injection. METHODS :The experiment is implemented according to the request of endotoxin inspction method clollected in the 2 nd book,CHINA pharmacopeia,1995. RESULTS :The experiment shows that the interference elements can be excluded by diluting norflovacin and gloucose injection 4 times,and it is effective that endotoxin can be tested by amebocyte lysate with accuracy of 0.125Euml-1. CONCLUSIONS :For daily hospital pyogen test of norflovacin and gloucose injection,amebocyte lysate method can be used instead of domestic rabbit method.