摘要
目的为证实人胚胎下丘脑是否存在褪黑素受体(MR),以及GTPγS和电解质对MR的影响,以阐明褪黑素(M)生物学特性及对内分泌系统作用的机制。方法应用放射配体结合法检测人胚胎下丘脑〔125I〕M特异结合。结果1.人胚胎下丘脑〔125I〕M特异结合的Scatchard分析:最大结合容量(Bmax)为(1.15±0.32)fmol/mg蛋白质;平衡解离常数(Kd)为(36±8)pmol/L;2.动力学分析表明为可逆性结合;3.特异性分析表明对M高度特异性;4.10μmol/L和50μmol/LGTPγS使〔125I〕M特异结合降低,且有剂量依赖性。结论实验结果表明人胚胎下丘脑存在〔125I〕M特异结合位点。GTPγS对〔125I〕M特异结合有明显抑制作用。
Objective To verify
whether there exists melatonin receptor in human embryonic hypothalamus. Methods
Specificbindingofmelatonintoembryonic hypothalamus was measured by radioligand binding
assay. Results Themaximumbindingcapacity(Bmax) was (1.15±0.32)fmol/mg protein and
equilibrium dissociation constant (Kd) was (36±8)pmol/L. GTPγS dose dependently inhibited
the binding. Conclusion The results demonstrate the presence of specific binding of melatonin
in human embryonic hypothalamus. GTPγS inhibits the binding, indicating that putative
melatonin receptor is coupled to G proteins.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1999年第3期149-152,共4页
Chinese Journal of Endocrinology and Metabolism