摘要
目的探讨内皮素１及其ｍＲＮＡ在早期实验性糖尿病大鼠肾脏中的含量和意义。方法采用免疫组织化学、原位杂交技术检测糖尿病大鼠肾组织中ＥＴ１及其ｍＲＮＡ。结果ＥＴ１及ＥＴ１ｍＲＮＡ分布一致，主要分布于肾小球内皮细胞、系膜细胞、血管平滑肌细胞及肾小管上皮细胞之中，髓质高于皮质，二者在糖尿病发生后１～２周肾小球内均明显减少（Ｐ＜０．０５），４周时开始增加（Ｐ＜０．０５），而在髓质中则持续增加。
Objective To observe the content of ET 1 and
its mRNA in kidney of diabetic rats at early stages and its significance. Methods Diabetes
mellitus was induced by an interperitoneal injection of streptozotocin (55mg/kg). The content of
ET 1 was examined by immunohistochemical stainning and then half quantitative analysis by
Mia 2000 pathology computer image analyser while the content of ET 1 mRNA was observed
by in situ hybridizational technique. Results The distribution of ET 1 and its mRNA were
similar. The postive signal of ET 1 and ET 1 mRNA were observed in glomerular endothelial,
mesangial, vascular smooth muscle and renal tubular epithelial cells. In glomeruli of the 1st
2nd weeks, ET 1 and ET 1 mRNA were decreased significantly compared with the normal
control (P<0.05). But in tubulointerstitial segment of diabetic rats, they were increased as
compared with the normal control. Conclusion ET 1 may play a role in the pathogenesis of
glomerular hyperinfusion and tubulointerstitial injury.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1999年第3期168-171,共4页
Chinese Journal of Endocrinology and Metabolism
