摘要
蛋白电泳方法具有电泳谱带清晰、分辨率高、品种问谱带差异大等优点,因而在作物种子纯度鉴定中具有重要意义。本文综述了蛋白电泳技术的原理、操作要点、影响因素及在电泳过程中常出现的问题及解决办法,旨在为从事种子鉴定的同行提供参考。
Salt-in protein electrophoresis technic has played an important role in identification of maize variety purity because of such characters as follows: shorter extraction time of sampleand shorter elec trophoresis time, legible electrophoresis bands, higher resolution,various bands between varieties and evi dent complementaryl bands between different combinations.the theory of identifiction of maize variety puri ty, key technics, analysis ways of electrophoresis bands, the problems tin the course of electrophoresis and the ways to resolve are reported in this paper. The aim is to supply help for craft brother.
出处
《牧草与饲料》
2010年第3期24-27,共4页
Journal of Forage & Feed
关键词
蛋白质电泳技术
种子纯度鉴定
应用
Protein electrophoresis technic
Identification of crop variety purity