摘要
为探讨视网膜的免疫原性,选择DBA/2鼠(MHC为H2d)为供体,C57BL/6(MHC为H2b)或BALB/C鼠(MHC为H2d)为受体,把DBA/2鼠的半个视网膜移植于受体鼠的皮下,2周后检测迟发型超敏反应(DTH)和细胞毒性T淋巴细胞(CTL)。结果示移植组的迟发型超敏反应(DTH)和细胞介导的细胞毒效应(CTL)均低于阳性对照DBA/2鼠脾细胞组(P<0.01),高于阴性对照假手术组(P<0.01)。提示DBA/2鼠视网膜表达MHCⅠ类和Ⅱ类抗原及MinorHC抗原,刺激受鼠产生较弱的细胞免疫应答。视网膜具有弱免疫原性。
Objective: To evaluate the immunogenicity of retina. Methods: DBA/2 mice (MHCH 2 d) were used as donors, C57BL/6 mice (MHC H 2 d) or BALB/C mice (MHC H 2 d) as recipients. Half retina of DBA/2 mouse was transplanted subcutaneously to a C57BL/6 or BALB/C mouse. The delayed type hypersensitivity (DTH) and specific killing rate of cytotoxic T lymphocyte(CTL) were detected two weeks later. Results: The results of DTH and specific killing rate of CTL in the retina of DBA/2 mice were significantly lower than the positive group( P <0.01), but significantly higher than the negative group( P <0.01). Conclusion: MHC Class Ⅰ and Class Ⅱ antigen and minor histocompatibility complex antigen(Minor HC) are expressed in the retina of the DBA/2 mouse, which stimulates immune system of recipient to produce weak cellular immune response. It is suggested that the retina has weak immunogenicity.
出处
《湖南医科大学学报》
CSCD
1999年第3期215-218,共4页
Bulletin of Hunan Medical University
基金
湖南省自然科学基金
关键词
视网膜
小鼠
免疫原性
视网膜移植
retina
transplantation
delayed type hypersensitivity *
cytotoxicity tests,immunologic
disease models,animal
mice