α-黑素细胞刺激素对实验性变态反应性脑脊髓炎防治作用的初步研究

Protective effect of alpha-melanocyte-stimulating hormone on experimental allergic encephalomyelitis in guinea pig

目的:探讨α-黑素细胞刺激素(α-MSH)对实验性变态反应性脑脊髓炎模型(EAE)的发病的保护作用。方法:40只雌性豚鼠随机分成正常对照组、EAE对照组、EAE低剂量治疗组和EAE高剂量治疗组,每组各10只。造模前7天开始干预,直至实验结束。记录发病豚鼠的潜伏期、进展期和高峰期神经功能障碍评分;检测外周血单个核细胞(PBMC)分泌IFN-γ、IL-4、IL-17及IL-23能力。结果:EAE高、低剂量治疗组虽均有发病,但潜伏期相对较长,神经功能障碍程度也相对较轻;α-MSH高、低剂量治疗组豚鼠PBMC分泌IFN-γ、IL-17、IL-23能力较EAE对照组降低(P<0.01),分泌IL-4能力较EAE对照组升高(P<0.01);高剂量治疗组改变较低剂量治疗组明显。结论:α-MSH对EAE豚鼠发病具有保护作用。

Objective：To investigate the prophylactic and treatment effects of alpha-melanocyte-stimulating hormone （alpha-MSH） in guinea pig model of experimental allergic encephalomyelitis （EAE）.Methods：40 female guinea pigs were assigned randomly to four groups： the normal group,the EAE control group,the low dose treating EAE group and the high dose treating EAE group.Intervention was conducted from 7d before the model establish to the experiment end.The delitescence,progression and fastigium neurological dysfunction score were recorded and the mortality of each group was compared.The level of IL-4,IL-17,IL-23,IFN-γ secreted by peripheral blood mononuclear cells （PBMC） were detected by ELISA.Results：There was no incidence of EAE in the normal group but various incidence occurred in the EAE control group,the low dose treating EAE group and the high dose treating EAE group.The progression period was relatively long and the neurological dysfunction score was also relatively light.The levels of IFN-γ,IL-17,IL-23 of α-MSH high dose treating and low dose treating group were lower than those of the EAE control group （P0.01）,but the level of IL-4 was opposite （P0.01）.The significantly changes existed in the high dose treating group.Conclusion：α-MSH has protective effect on the incidence of EAE,which is positively correlated with the dose of α-MSH.