3-硝基-5-(6-溴己酰胺)苯甲酸的合成及其作为头孢菌素酰化酶产生菌筛选指示剂的研究

The synthesis of 3nitro5(6bromohexanoylamino) benzoic acidand the studies on its use as an indicator ofcephalosporin Cacylaseproducingmicroorganisms

为筛选头孢菌素Ｃ酰化酶产生菌，由环己酮和３－硝基－５－乙酰胺基苯甲酸出发，合成了一种新的生色物质３－硝基－５－（６－溴己酰胺）苯甲酸（３－Ｎｉｔｒｏ－５－（６－ｂｒｏｍｏｈｅｘａｎｏｙｌａｍｉｎｏ）ｂｅｎｚｏｉｃａｃｉｄ，ＮＢＨＡＢ）。通过红外光谱法、核磁共振法、质谱法鉴定了其结构。ＧＬ－７ＡＣＡ酰化酶和青霉素Ｇ酰化酶（ｐｅｎｉｃｉｌｉｎＧＡｃｙｌａｓｅ，ＰＧＡ）及其产生菌对该化合物的作用表明，该物质能够检测头孢菌素酰化酶活力，用来筛选头孢菌素Ｃ酰化酶产生菌，其灵敏性有待进一步提高。

For screening the microorganisms capable of producing cephalasporin C acylase, 3nitro5(6bromohexanoylamino) benzoic acid, a new chromogenic substrate, was prepared with the starting material cyclohexanone and 3nitro5acetamidobenzoic acid. Its structure and constitution were characterized by IR, 1 HNMR and MS. The attemptation using this substrate as an indicator to detect cephalosporinCacylaseproducingmicroorganisms was carried out. This substrate can detect the activity of cephalosporin acylase, its sensitivity should be improved if using it as an indicator of cephalosporin Cacylaseproducingmicroorganisms.