浸透限制固定相高效液相色谱法测定人血浆中头孢唑林、头孢曲松、头孢唑肟的浓度

Determination of cefazolin, ceftriaxone and ceftizoximein plasma by high performance liquid chromatographywith restricted access stationary phases

目的：建立一种直接进样高效液相色谱法测定人血浆中的头孢唑林、头孢曲松、头孢唑肟的血药浓度。方法：选用浸透限制固定相，紫外检测器，使３种头孢菌素分别在流动相ｐＨ５．７５、ｐＨ５．２５和ｐＨ４．３５条件下与内标及血浆中的蛋白质进行良好的分离。结果：制作了３种头孢菌素各自的标准曲线，测定方法的变异系数在１．２％～７．１％，回收率近１００％。最后，实测了６例临床静脉输注头孢唑林、头孢曲松、头孢唑肟病人的血药浓度。结论：浸透限制固定相色谱法测定血浆中的头孢菌素，省去了前处理，快速简单，更适用于临床治疗药物监测。

Objective: To determine cefazolin, ceftriaxone and ceftizoxime in plasma by high performance liquid chromatographic direct injection. Methods: Three kinds of cephalosporins in plasma were determined respectively by HPLC with restricted access stationary phases and UV detection. Cefazolin, ceftriaxone and ceftizoxime were well separated from internal standard and proteins in pH5.75, pH5.25 and pH4.35. Results: The standard curve of every cephalosporin was calculated. The average recovery rate was almost 100% and the coefficients of variation were 1.2%￣7.1%. The plasma of 6 patients after iv cefazolin or ceftriaxone or ceftizoxime was analysed. Conclusions: High performance liquid chromatography with restricted access stationary phases without any pretreatment may be used to routine clinical use. This is a simple and rapid method.