2,4-D对盐藻生物量与物质积累的影响

Effects of 2,4-D on the biomass and nutrient accunulation of Dunaliella salina

为了研究2,4-D对杜氏盐藻生物量和物质积累的影响,试验采用向对数生长期的杜氏盐藻(Dunaliella salina)中分别添加浓度为0.01,0.05,0.1,0.5,1,5,10,25,50 mg/L植物激素2,4-D对其进行诱导培养,周期性收集藻细胞,对各阶段藻细胞中细胞密度、β-胡萝卜素、叶绿素、总可溶性蛋白含量进行检测和比较。结果表明:在1个月的诱导期内,用0.1 mg/L 2,4-D处理不但能显著加快藻细胞的分裂速度,而且比对照组的生物量提高了22%,β-胡萝卜素的产量增加了7.8%;但该处理的叶绿素含量、总可溶性蛋白含量与其他处理组及对照组都没有明显的区别。

To study the effect of extraneous 2,4-D on the growth and biochemical composition of Dunaliella salina,different concentrations of 2,4-D were added into the vegetative green cells.In this stage,cell density,β-carotene,chlorophyll and total soluble proteins contents were determined respectively.The results showed that cell density and β-carotene production of 0.1 mg/L 2,4-D treatment were 22% and 7.8% higher than those of the control.Chlorophyll and total soluble proteins contents of this treatment had no evident difference from those of other treatments and the control.