猪牙花乙醇提取物抑菌活性、抗氧化作用及猪牙花中微量元素的测定

Antibacterial and Hydroxyl Radical Scavenging Activities and Trace Element Determination of Ethanol Extract from Erythronium japonicum Decne

目的:对猪牙花乙醇提取物的抑菌活性、抗氧化作用进行研究,并测定猪牙花中微量元素的含量。方法:以猪牙花全草80%乙醇提取物为材料,采用滤纸片法对4种常见细菌的生长抑制活性、最低抑菌浓度进行测定,并讨论温度、pH值及紫外线对提取物抑菌效果的影响;采用羟自由基法测定其抗氧化作用;采用原子吸收分光光度法测定其微量元素铁、锰、锌、铜的含量,采用SPSS16.0统计分析软件对实验结果进行统计分析。结果:猪牙花全草80%乙醇提取物对金黄色葡萄球菌的最小抑菌质量浓度为12.5mg/mL,对沙门氏菌和枯草芽孢杆菌的最小抑菌质量浓度均为100mg/mL,对大肠杆菌的最小抑菌质量浓度为200mg/mL。热处理、酸性环境、紫外照射等对抑菌作用均无明显影响。猪牙花提取液在0.0025～0.32mg/mL的质量浓度范围内,其清除率达到64.57%。其提取物IC50值为0.051mg/mL。微量元素Fe含量为633.25μg/g;Mn为30.45μg/g;Zn为39.95μg/g;Cu为12μg/g。结论:猪牙花80%乙醇提取物有着良好的抑菌效果及较强的抗氧化作用,且猪牙花中微量元素含量较高,具有很大的开发价值。

Objective：To evaluate whether the ethanol extract from Erythronium japonicum Decne has antibacterial activity and the ability to scavenge hydroxyl radicals and determine its trace elements.Methods：The antibacterial activity of the ethanol extract against four common bacteria was assessed by measuring its minimal inhibitory concentrations （MIC） by filter paper method and the effects of temperature,pH and UV irradiation on its antibacterial activity were dealt with.Trace elements such as iron,magnesium,zinc and copper were determined using atomic absorption spectrophotometry.Experimental results were subjected to statistical analysis using SPSS16.0.Results：The MICs of the ethanol extract were 12.5,100,100 mg/mL and 200 mg/mL for Staphylococcus aureus,Salmonella,Bacillus subtilis and Escherichia coli,respectively.Thermal treatment,acidic environment and UV irradiation had no obvious effect on the antibacterial activity of the ethanol extract.The contents of iron,magnesium,zinc and copper of the ethanol extract were 633.25,30.45,39.95μg/g and 12μg/g,respectively.Conclusion：Erythronium japonicum Decne contains high levels of trace elements and exhibits excellent antibacterial and hydroxyl radical scavenging activities.Thus,this herb has a high exploitation value.