摘要
采用SDS-PAGE、Urea-PAGE分析耐热蛋白酶对UHT乳蛋白的水解作用。结果表明:荧光假单胞菌(PF)蛋白酶和纤溶酶(PL)作用底物不同,产物也不同;前者优先水解κ-酪蛋白生成副κ-酪蛋白,而PL主要水解β-酪蛋白与α-酪蛋白生成γ-酪蛋白及胨、肽等。分别经PL和细菌蛋白酶水解3h的UHT乳水解产物,在pH4.6和12g/100mL三氯乙酸(TCA)沉淀后经反相高效液相色潽仪(RP-HPLC)分析滤液,两者呈现不同的色谱图。
The effect of heat-resistant protease on protein proteolysis of UHT milk was evaluated by SDS-PAGE and UreaPAGE.Results indicated that different proteases reacted with different substrates in milk to produce different peptides.Pseudomonas fluorescens protease hydrolyzed κ-casein into para-κ-casein.Plasminogen hydrolyzed α-casein and β-casein into γ-casein,peptone and peptides.In addition,the leftover supernatants after trichloroacetic acid precipitation of the 3 h hydrolysates of UHT milk prepared with plasminogen and bacterial proteases exhibited different RP-HPLC profiles and those of the pH 4.6 soluble peptide in the 3 h hyolroluysates were also different.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第17期228-231,共4页
Food Science
基金
国家科研院所社会公益项目(2002005DIA4G035-7)
"十一五"国家科技支撑计划项目(2006BAD04A07
2006BAD04A10)
关键词
耐热蛋白酶
UHT乳
水解
heat-resistant protease
UHT milk
proteolysis
