摘要
通过对灌胃牛乳铁素(LfcinB)后小鼠血清中细胞因子IL-2、IL-4、IL-6、IL-10和TNF-α水平的变化,评价其免疫调节作用。结果表明,灌胃牛乳铁素后3h采血,分离其血清,用ELISA试剂盒检测细胞因子,与阴性对照组相比,对细胞因子IL-2和TNF-α产生极显著促进作用(P≤0.01),对抗炎细胞因子IL-4有极显著的抑制作用(P≤0.01),对IL-6有显著的抑制作用(P≤0.05),对抗炎细胞因子IL-10的分泌影响也极显著(P≤0.01),并且IL-10随TNF-α的增强而增强,起到平衡作用。因此,口服牛乳铁素对实验小鼠具有免疫增强和调节作用。
In order to figure out whether bovine lactoferricin （LfcinB） has immuno-regulation function,cytokines such as IL-2,IL-4,IL-6,IL-10 and TNF-α in blood sera from mice orally administered with LfcinB were tested using ELISA kits.The results indicated that IL-2 and TNF-α levels in lactoferricin treatment mice at 3 h post-administration were both even higher （P ≤ 0.01） than those in negative control mice,whereas the levels of anti-inflammatory cytokines,IL-4 （P ≤ 0.01） and IL-6 （P ≤ 0.05） revealed a significant decrease,and IL-10 level which is positively correlated with TNF-α level and plays a balancing role also changed notably.Therefore,oral administration of lactoferricin has the potential to provide the function of immune enhancement and regulation.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第17期245-249,共5页
Food Science
基金
天津市科技支撑计划重点项目(07ZCKFNC00200)
关键词
牛乳铁素
细胞因子
免疫调节
lactoferricin
cytokine
immuno-regulation function
