摘要
采用ERIC-PCR技术对12株秀珍菇菌株的亲缘关系进行分析,并与拮抗实验和酯酶同工酶实验进行对比。结果表明:在12株秀珍菇菌株中,ERIC-PCR扩增出的条带清晰,重复性好,多态性高。PCR产物大致在150～3500bp范围内,各菌株扩增条带数在9～16条之间。聚类分析表明,在79%的分类水平上,12个菌株聚成4组。MY灰和MY白菌株亲缘关系较近,聚为第1组;HZ845为第2组;HZ3、HZ5和HZ夏亲缘关系很近,聚为第3组;LD1、GY16、GY18、GY163、SM、XY秀1亲缘关系很近,聚为第4组。ERIC-PCR聚类分析结果与拮抗实验和酯酶同工酶分析结果基本一致,验证了ERIC-PCR技术的可靠性。
ERIC-PCR technique was used to analyze the phylogenetic relationships among 12 kinds of Pleurotus pulmonarius strains.The results indicated that the amplified product bands of ERIC-PCR were mainly distributed in a range from 150 to 3500 bp with good reproducibility and high polymorphism.The number of amplified bands varied from 9 to 16 for each strain.Cluster analysis revealed 79% similarity and 4 distinctive clusters among 12 strains.Based on the close phylogenetic relationship,MY Gray and MY White were designated as the first cluster,and HZ845 was designated as the second cluster,and the third cluster included HZ3,HZ5 and HZ Xia,and the fourth cluster included LD1,GY16,GY18,GY163,SM and XY-Xiu1.Meanwhile,the third cluster and the fourth cluster exhibited much closer phylogenetic relationship.The tested phylogenetic relationships among P.pulmonarius strains by ERIC-PCR were consistent with the results from antagonistic test and isozyme analysis of esterase.Therefore,ERIC-PCR is reliable in the identification of P.pulmonarius strains.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第17期267-271,共5页
Food Science
基金
"十一五"国家科技支撑计划项目(2008BADA1B04)
山东省"泰山学者"建设工程专项经费项目(鲁发[2003]20号)
鲁东大学引进人才博士基金项目(LY2010003)
关键词
ERIC-PCR技术
拮抗实验
酯酶同工酶
秀珍菇
亲缘关系
ERIC-PCR technique
antagonistic test
esterase isozyme
Pleurotus pulmonarius
phylogenetic relationship
