螺旋藻多糖通过腹腔黏膜对小鼠产生的免疫调节作用

Spirulina platensis Polysaccharides Exert Immunoregulatory Effect through the Mucous Membranes of the Abdominal Cavity

目的:分析螺旋藻多糖3种不同给药途径对小鼠血清中24种细胞因子的影响,进而研究螺旋藻多糖对小鼠的免疫调节作用。方法:通过灌胃、腹腔注射、尾静脉注射3种途径分别给小鼠不同剂量的螺旋藻多糖,对照组给与实验组同等体积的生理盐水。灌胃组在3h后眼球取血,腹腔注射和尾静脉注射组在2h后眼球取血,分离血清,采用Millipore小鼠细胞因子试剂盒和液体芯片扫描仪检测血清中24种细胞因子的浓度。结果:3种给药方式小鼠的IL-1α、IL-1β、IL-6、TNF-α、IFN-γ等炎症细胞因子都有不同程度的显著性升高,说明螺旋藻多糖对炎症细胞因子的分泌和先天免疫有促进作用。其中,腹腔注射螺旋藻多糖对细胞因子的影响最为显著,这种方式的信号传导途径主要包括NF-κB、MAPK、JAK/STAT等。结论:从螺旋藻多糖对小鼠细胞因子的影响来看,总体上表现出促进免疫的作用,推测螺旋藻多糖主要是通过对肠黏膜系统的受体相互作用,刺激相应细胞产生细胞因子来发挥其免疫调节作用和多种生理功能的。

Objective：To analyze the effects of three different administration routes of Spirulina platensis polysaccharides on 24 cytokines in mouse serum,followed by understanding of the immunoregulatory effect of Spirulina platensis polysaccharides.Methods：Three administration routes used were oral administration,intraperitoneal injection and intravenous injection at different doses each.Mice orally administered the same volume of normal saline were served as a control group.Serum was separated from eye ball at 3 h after administration for oral administration and at 2 h after administration for intraperitoneal injection and intravenous injection.The contents of 24 cytokines in mouse serum were examined using Milliplex map kit or luminex xMAP technology.Results：Compared with the control group,each treatment group showed significantly higher serum IL-1α,IL-1β,IL-6,TNF-α and IFN-γ contents,indicating that Spirulina platensis polysaccharides have an anxo-action on inflammation cytokines.Among the three administration routes,the effect of intraperitoneal injection on cytokines was the most obvious,and the cell signaling mode of this route mainly consisted of NF-κB,MAPK and JAK/STAT.Conclusion：In general,Spirulina platensis polysaccharides have immunopromoting effect on cytokines,and their immunoregulatory and multiple physiological functions may be exerted through interactions among intestinal mucous membrane receptors and exciting the secretion of cytokines by homologous cells.