摘要
通过硫酸铵分级沉淀、DEAE Sepharose Fast Flow柱层析、Sephadex G-200柱层析,以小麦蠕孢病菌和金黄色葡萄球菌为指示菌采用抑菌活性追踪和SDS-PAGE跟踪检测,从分离自连云港海域、对多种病原真菌具有抑菌作用的多黏类芽孢杆菌L1-9菌株发酵液中分离纯化得到一种对金黄色葡萄球菌和小麦蠕孢病菌的菌丝生长及孢子萌发具有抑制作用的抗菌蛋白,分子质量约31kD。
An antimicrobial protein was obtained from the fermentation broth of a Paenibacillus polymyxa strain isolated from the sea area near Lianyungang (numbered L1-9),which has antagonic effect against a variety of pathogenic fungi,after ammonium sulfate precipitation and DEAE Sepharose Fast Flow ion exchange and Sephacryl G-200 filtration chromatographic separation.This protein was found to have the ability to inhibit the growth of Staphyloccocus aureus and the mycelial growth and spore germination of Helminthosporium sativum and possess a molecular weight of about 31 kD.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第17期335-339,共5页
Food Science
基金
江苏省自然科学基金项目(BK2009165)
连云港市科技局农业攻关项目(CN0825)
农业部生物防治重点开放实验室开放课题(KLBC-09-01)
江苏省海洋生物技术重点建设实验室开放课题(HS08009)