摘要
利用251个株系组成的Maybelle/白叶秋的加倍单倍体群体,构建了由226个SSR分子标记组成的遗传图谱。通过两年抗旱棚苗期抗旱性鉴定,应用复合区间作图法和QTLNetwork2.0对水稻苗期抗旱性进行QTL定位及互作效应分析。利用前者在两年共检测到5个抗旱性相关QTL,分别位于第2、3、5、6和8染色体;而通过后者在第2、3、5和6染色体上也找到了抗旱性相关的QTL,并且通过两种方法检测到的第3、5、6染色体上的3个QTL所在区间吻合;还发现4个具有上位性的QTL。所有抗旱性QTL的加性效应均为正值,表明来自父本白叶秋的这些抗旱性位点可以提高水稻的抗旱性。
The QTLs for drought resistance at the seedling stage were analyzed by using a doubled haploid （DH） population consisted of 251 lines from the cross between a japonica parent Maybelle and an indica parent Baiyeqiu. A genetic linkage map with 226 SSR markers was constructed. Through single-locus analysis following composite interval mapping （CIM）,a total of 5 QTLs were detected on 5 different chromosomes. Four QTLs were also detected by two-locus analysis,which resolved 4 epistatic QTLs （E-QTLs）. The additive genetic effects of all QTLs were positive,indicating the alleles from Baiyeqiu improve drought tolerance of the DH population.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2010年第5期469-473,共5页
Chinese Journal of Rice Science
基金
中央级公益性科研院所专项资金资助项目(2009RG001-3)
浙江省农业重大科技项目(2007C12073)
国家科技支撑计划资助项目(2006BAD13B01-5)
关键词
水稻
抗旱性
加倍单倍体群体
数量性状基因座
rice
drought resistance
doubled haploid population
quantitative trait locus
