免疫性抗体对女性不孕不育检测的临床意义

Clinical Significance of Immune Antibody Testing in Female Infertility

目的探讨抗精子抗体(AsAb)、抗子宫内膜抗体(EMAb)、抗心磷脂抗体(AcL)检测在女性不孕不育中的意义。方法采用ELISA法检测1 125例女性不孕不育患者进行血清中AsAb、EMAb、AcL抗体,同时与健康组作对比研究。结果不孕不育组抗精子总抗体(AsAb-T)的阳性率为60.1%(676/1 125),健康组抗精子总抗体阳性率为5.2%,两组差异有统计学意义(P<0.01);EMAb检测不孕不育组总阳性率为32.5%(366/1 125),健康组阳性率为5.3%,两组对比,差异有统计学意义(P<0.01);AcL检测不孕不育组总阳性率为22.2%(250/1 125),健康组阳性率为4.1%,两组对比,差异有统计学意义(P<0.05)。AsAb、EMAb、AcL检测中,其中两项同时阳性的,不孕不育组有204例(18.1%),三项同时阳性的,不孕不育组有103例(9.2%);健康组无两项、三项同时阳性的。结论 AsAb、EMAb、AcL是引起女性不孕不育的重要免疫因素,不孕不育者应进行免疫性抗体的检测。

Objective To investigate the significance of testing antisperm antibody（AsAb）,antiendometrium antibody（EMAb）,anticardiolipin antibody（AcL） in female infertility. Methods The serum AsAb,EMAb,and AcL were detected in 1,125 female patients who suffered from infertility and 75 healthy women by ELISA method.The results of the two groups were compared and analyzed. Results The total masculine rates of AsAb of infertility group and healthy control group were 60.1%（676/1,125） and 5.2%,respectively;and the difference was statistically significant between the two groups（P0.01）.The total masculine rates of EMAb of infertility group and healthy control group were 32.5%（366/1,125） and 5.3%,respectively;and the difference was statistically significant between the two groups（P0.01）.The total masculine rates of AcL of infertility group and healthy control group were 22.2%（250/1,125） and 4.1%,respectively;and the difference was statistically significant between the two groups（P0.05）.Among the testing of AsAb,EMAb and AcL,there were 204（18.1%） patients who were masculine in two items and 103（9.2%） patients who were masculine in three items.The healthy group had no absolutely masculine. Conclusions AsAb,EMAb,and AcL are the important immune factors in female infertility,and the patients should strengthen the test of immune antibody in couples.