Galectin-3对骨髓间充质干细胞来源的内皮细胞增殖的影响

Influence of galectin-3 on proliferation of endothelial cells induced from bone marrow mesenchymal stem cells

目的探讨galectin-3对骨髓间充质干细胞(MSCs)来源的内皮细胞增殖的影响。方法密度梯度离心法分离纯化SD大鼠骨髓MSCs,取第3代,加入血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF),体外诱导14 d,进行免疫组织荧光染色和电镜鉴定,用低、中、高不同浓度galec-tin-3(0.1μg/mL,1.0μg/mL和5.0μg/mL)对骨髓MSCs来源的内皮细胞作用24 h;采用MTT比色法观察不同浓度galectin-3对内皮细胞增殖的影响;利用流式细胞仪检测DNA细胞周期的变化。结果 (1)中高浓度galectin-3具有显著的促进血管内皮细胞增殖的作用(P<0.05);(2)流式细胞仪检测DNA细胞周期显示各组S期细胞明显增加(P<0.05),中高浓度galectin-3组G2M期细胞比例明显增加(P<0.05)。结论 galectin-3可以促进骨髓MSCs来源的内皮细胞增殖。

Objective To observe the effects of galectin-3 on proliferation of endothelial cells induced from bone marrow mesenchymal stem cells.Methods The cultured mesenchymal stem cells in vitro were isolated and purified from Sprague-Dawley rats bone marrow by gradient centrifugation on lymphocytes separation medium.Cells of passage 3 were differentiated into endothelial cells by vascular endothelial growth factor（VEGF）and basic fibroblast growth facotr（bFGF）.These cells were identified as endothelial cells by immunohistochemistry stain and electronic microscopy 14 days after induction.Then,the cells were cultivated with the galectin-3 for 24 hours with centrations of 0.1μg/mL,1μg/mL and 5μg/mL.The proliferation of endothelial cells induced from bone marrow mesenchymal stem cells was measured by MTT,and flow cytometry was applied to observe cell cycle.Results（1）Moderate and high concentrations（1μg/mL and 5μg/mL） of galectin-3 promoted proliferation of endothelial cells（P0.05）;（2） Flow cytometry showed that S phase cells increased obviously in every group（P0.05）and G2M phase increased in the concentrations of 1μg/mL and 5μg/mL（P0.05）.Conclusions Galectin-3 can promote the proliferation of endothelial cells induced from bone marrow mesenchymal stem cells.