盐酸氟西汀对抑郁模型大鼠胃窦肥大细胞胰蛋白酶-1的影响

Effects of Fluoxetine Hydrochloride on Mast Cell Protease-1 Expression in Gastric Antrum of Depression Model Rats

目的:观察盐酸氟西汀对抑郁模型大鼠胃窦黏膜肥大细胞胰蛋白酶-1(MCP-1)的影响。方法:将慢性、不可预见性、非同质性应激原作为抑郁模型,通过免疫荧光组织化学及RT-PCR技术研究盐酸氟西汀对抑郁模型大鼠胃窦黏膜MCP-1分泌的影响。结果:和正常对照组相比,抑郁模型组和生理盐水+抑郁模型组大鼠胃窦黏膜组织MCP-1平均免疫荧光强度值分别为(37.4±7.7)和(39.9±5.0),显著高于正常对照组(24.8±5.6)(P<0.01);盐酸氟西汀+正常对照组和盐酸氟西汀+抑郁模型组分别为(23.1±3.4)和(26.1±3.6),与正常对照组差异不明显(P>0.05)。抑郁模型组和生理盐水+抑郁模型组大鼠胃窦黏膜组织MCP-1mRNA表达分别为(0.759±0.357)和(0.781±0.451),显著高于正常对照组(0.476±0.029)(P<0.01);盐酸氟西汀+正常对照组和盐酸氟西汀+抑郁模型组分别为(0.460±0.027)和(0.488±0.030),与正常对照组差异不明显(P>0.05)。结论:盐酸氟西汀对抑郁模型大鼠具有下调胃窦部免疫反应的作用,对胃窦部具有保护作用。

Objective：To investigate the effect of fluoxetine hydrochloride on mast cell protease-1 （MCP-1）expression in depression model rats.Methods：The chronic stressed depression rat models were established by chronic unpredictable mild stress.Laser scanning confocal microscopy （LSCM）immunofluorecence and RT-PCR techniques were used to investigate MCP-1 expression in gastric antrum.Results：Fluoxetine was observed to play part inhibition effects on mast cells ultrastructure alterations.Compared to the normal control group,the average immunofluorescence intensity of gastric antrum MCP-1 significantly increased in chronic stress-induce depressed model group（37.4±7.7）or saline＋depressed model group（39.9±5.0）（P0.01）,while there was no significant difference between fluoxetine＋normal control group（23.1±3.4） or fluoxetine＋depressed model group（26.1±3.6）and normal control group（24.8±5.6）.The average level of rMCP-1 mRNA in gastric antrum significantly increased in chronic stress-induce depressed model group（0.759±0.357）or saline＋depressed model group（0.781±0.451）（P0.01）,while there was no significant difference between fluoxetine＋ normal control group （0.460±0.027）or fluoxetine＋ depressed model group（0.488±0.030）and normal control group（0.476±0.029）.Conclusion：Fluoxetine hydrochloride had negative immunoregulatory effects and might play aprotective role on gastric antrumin in depression model rats.