川芎嗪对幼兔肺缺血再灌注损伤的保护作用

Protective Effect of Ligustrazine on Lung Ischemia-Reperfusion Injury in Immature Rabbits

目的:探讨川芎嗪(LZ)对幼兔肺缺血再灌注损伤中肺的保护作用。方法:将36只实验用幼兔随机分成缺血再灌注组(I/R)、假手术组(S)和川芎嗪组(LZ60mg/kg),每组12只。建立在体幼兔肺缺血再灌注模型,并检测肺组织中超氧化物歧化酶(SOD),丙二醛(MDA)及髓过氧化物酶(MPO)的水平。测定肺泡灌洗液(BALF)中性粒细胞(PMN)计数、计算肺湿干重比(W/D)和光镜下观察肺组织病理改变。结果:I/R组与S组相比,SOD活性下降,W/D、MDA含量及MPO活性均显著增加,肺组织病理损伤明显。LZ组与I/R组相比,W/D、SOD活性增加,MDA含量、MPO活性降低及再灌注肺BALF中性粒细胞计数LZ组低于I/R组(P<0.05),肺组织病理损伤明显减轻。结论:川芎嗪能明显减轻幼兔肺缺血再灌注损伤,其作用机制可能与抑制中性粒细胞在肺内积聚、减轻氧自由基造成的肺损伤有关。

Objective：To investigate the effect of ligustrazine on lung ischemia-reperfusion（I/R）injury in immature rabbits.Methods：Thirty-six healthy experimental rabbits（aged 3-4 weeks old, weighing 350-450g）were randomly divided into three groups（n=12 in each）.The I/R group re-ceived ischemia（60min）and reperfusion（120min）in the left lung,while the ligustrazine group received ligustrazine（60mg/kg）intravenous injection before I/R.Polymorphonuclear leucocytes （PMN）count of bronchoalveolar lavage fluid（BALF）,malondialdehyde（MDA）,superoxide dismutase（SOD）,and myeloperoxidase（MPO）levels in lung tissue were determined.The changes of lung wet to dry weight ratio（W/D）and histological appearances were observed.Results：In I/R group,the activity of SOD decreased,W/D,levels of MDA and MPO,and PMN count of bronchoalveolar lavage fluid（BALF）were significantly higher than those in S group（all P0.05）,but the these changes had been alleviated significantly in LZ group.Conclusion：Ligustrazine could protect lung tissue from ischemia-reperfusion injury;the protective mechanism may be that ligustrazine can effectively decrease the PMN sequestration in the lung and alleviate the lung injury resulting from oxygen derived free radical.