淋巴组织免疫组织化学染色方法的探讨

Modified Methods for Immunohistochemistry Staining of Lymphoid Tissues

目的:鉴于病理淋巴组织的切片常见组织较硬、细胞脆性大,免疫组织化学染色显色模糊,甚至出现假阴性现象,探讨淋巴组织制片与免疫组化染色的新方法与新思路。方法:采用B-5固定液、软化剂的处理进行石蜡切片、免疫组织化学染色方法进行染色。结果:制出背景清晰、定位准确、反差分明、效果较好的免疫组化染色淋巴组织切片。结论:经B-5固定液固定、软化剂处理过的淋巴组织免疫组化切片能为淋巴组织疾病诊断提供定位准确、阳性明显的抗原表达信息。

Objective：To investigate a new standard experimental method for making paraffin-embedded sections and staining of immunohistochemistry in lymphoid tissues.Methods：The procedures for B-5 stationary liquid fixation and treatment with softener,paraffin embedding,cutting into sections and staining of immunohistochemistry of lymphoid tissues were described in detail.Results：The sections by this method revealed the more accurate location,markedly characteristic contrast, weaker background with good visibility,more detail and clear structure of tissues and cells,and were suitable for microphotography.Also staining results by this method were stable and reliable with strong specialicity and high sensitivity.Conclusion：This method can be applied as a new standard experimental method to make paraffin embedded sections and staining of immunohisto-chemistry in lymphoid tissues to enhance antigen presentation in lymphoid tissues.