人脑胶质瘤中DLL4表达与血管生成的关系

Delta-Like Ligand 4 Expression in Human Gliomas and Its Relationship with Angiogenesis

目的:分析Notch信号配体——Delta样配体4(DLL4)在人脑胶质瘤中表达及其与肿瘤恶性程度、微血管密度(MVD)之间的关系,探讨DLL4在肿瘤血管生成中的作用,及DLL4作为抗肿瘤治疗靶点的可能性。方法:免疫组织化学方法检测36例人脑胶质瘤及7例正常脑组织中DLL4和血管内皮生长因子(VEGF)表达情况,用CD31标记血管内皮细胞以计算MVD。结果:DLL4及VEGF表达水平在正常脑组织、高度恶性和低度恶性胶质瘤组两两之间均有差异(P<0.01),其表达随胶质瘤恶性程度升高而增强(P<0.01);DLL4和VEGF表达之间呈正相关(r=0.539,P<0.01),两者均与MVD相关。结论:DLL4在胶质瘤的血管生成中发挥重要作用,有望成为肿瘤抗血管生成治疗的靶点。

Objective：To investigate the relationship of Delta-like ligand 4（DLL4）expression with the malignancy and microvessel density（MVD）in human brain gliomas,and to explore the role of DLL4in the process of glioma angiogenesis and the possibility as target of antiangiogenic therapy.Methods：The expression of DLL4 and VEGF were detected in 38cases of human brain gilomas and 7normal brain tissues using immunohistochemical staining.MVD was calculated through CD31 staining for microvessel endothelium.Results：The expression of DLL4 in human brain gliomas was significantly up-regulated（P0.01）,and correlated with tumor malignant progress（P0.01）.DLL4 expression was associated with VEGF expression and MVD.Conclusion：DLL4 might play a key role in human glioma angiogenesis and is a novel target for antiangiogenic therapy.