宫颈癌组织中Survivin、Fas和FasL的表达及其关系

Expression of Survivin,Fas and FasL in cervical cancer and their relationship

目的检测不同宫颈病变组织中凋亡相关蛋白Survivin、Fas、FasL的表达,分析宫颈癌组织中Survivin与Fas/FasL表达的关系。方法采用免疫组织化学SP法检测47例宫颈癌、25例宫颈上皮内瘤样变(CIN)、20例慢性宫颈炎及20例正常宫颈组织中Survivin、Fas和FasL的表达,分析Survivin、Fas和FasL表达与宫颈癌临床病理特征的关系。采用Spearman秩相关法分析宫颈癌组织中Survivin与Fas/FasL表达的关系。结果 Survivin在宫颈癌组织中的阳性表达率(68.1%)明显高于正常宫颈组织(0%)、慢性宫颈炎组织(15.0%)和CIN组织(36.0%),差异均有统计学意义(P<0.05);Fas在宫颈癌组织中的阳性表达率(48.9%)明显低于正常宫颈组织(90.0%)、慢性宫颈炎组织(85.0%)和CIN组织(80.0%),差异均有统计学意义(P<0.05);FasL在宫颈癌组织中的阳性表达率(93.6%)明显高于正常宫颈组织(0)、慢性宫颈炎组织(50.0%)和CIN组织(68.0%),差异也均有统计学意义(P<0.05)。Survivin的表达与宫颈癌临床分期、是否有淋巴结转移有关(P<0.05);FasL的表达与宫颈癌的临床分期、病理分级、是否有淋巴结转移有关(P<0.05)。宫颈癌组织中,Survivin与Fas的表达呈负相关(r=-0.517,P<0.01),与FasL的表达呈正相关(r=0.381,P<0.01)。结论宫颈癌能通过Survivin和FasL高表达及Fas低表达逃避机体免疫监视,促使肿瘤发生、发展及转移。FasL可作为宫颈癌患者病程进展和预后的预测指标之一。

Objective To investigate the expression of Survivin,Fas and FasL in tissues of different cervical lesions,and analyse the relationship between the expression of Survivin and that of Fas/FasL in tissues of cervical cancer.MethodsThe expression of Survivin,Fas and FasL in tissues of 47 cases of cervical cancer,25 cases of cervical intraepithelial neoplasia （CIN）,20 cases of chronic cervicitis and 20 cases of normal cervix was detected by immunohistochemical staining （SP method）,and the relationship between the expression of Survivin,Fas and FasL and clinical pathologic characteristics of cervical cancer was analysed.Besides,the relationship between the expression of Survivin and that of Fas/FasL in tissues of cervical cancer was explored by Spearman rank correlation analysis.Results The positive expression rate of Survivin was 68.1% in tissues of cervical cancer,which was significantly higher than those in tissues of normal cervix （0%）,chronic cervicitis （15.0%） and CIN （36.0%） （P0.05）.The positive expression rate of Fas was 48.9% in tissues of cervical cancer,which was significantly lower than those in tissues of normal cervix （90.0%）,chronic cervicitis （85.0%） and CIN （80.0%） （P0.05）.The positive expression rate of FasL was 93.6% in tissues of cervical cancer,which was significantly higher than those in tissues of normal cervix （0%）,chronic cervicitis （50.0%） and CIN （68.0%）（P0.05）.The expression of Survivin was correlated with clinical staging and lymphnode metastases of cervical cancer （P0.05）.The expression of FasL was correlated with lymphnode metastases,clinical staging and pathological grading of cervical cancer （P0.05）.The expression of Survivin was negatively correlated with that of Fas in tissues of cervical cancer （r=-0.517,P0.01）,and was positively correlated with that of FasL （r=0.381,P0.01）.Conclusion The up-regulation of expression of Survivin and FasL and down-regulation of expression of Fas may take part in the carcinogenesis and development of cervical cancer,which may be the mechanism by which tumor cells evade the immune surveillance.The expression of FasL may be one of the prediction indexes for disease progression and prognosis in cervical cancer.