双酚A对雄性小鼠睾丸发育和生殖激素分泌水平的影响

Effects of bisphenol A on development of testis of adult male mice and on levels of their testosterone and luteinizing hormone

为研究不同剂量的双酚A(BPA)对雄性小鼠生殖系统的损伤作用,将25g左右的雄性小鼠40只随机分成对照组、BPA(10、50、100mg/kg)染毒组,每组10只,连续7d对小鼠腹腔注射BPA,对照组注射等量的橄榄油,1次/d,染毒结束后摘取并称量大鼠睾丸、附睾质量并计算脏器指数;光镜检测睾丸病理学改变;酶联免疫法检测血中睾丸酮、黄体生成素水平。结果显示,与对照组相比,小鼠睾丸重量、附睾重量及其脏器指数显著降低(P<0.05,P<0.01);双酚A组中的曲细精管部分支持细胞与生精细胞分离,生精细胞松散,无规律,各层细胞发生紊乱;随染毒剂量的增加,小鼠血清中睾丸酮和黄体生成素的含量逐渐降低,50mg/kg和100mg/kg两个剂量组睾丸酮和黄体生成素的浓度与对照组相比差异极显著(P<0.01)。结果表明BPA可引起雄性小鼠生殖系统的损伤。

To study the effects of bisphenol A（BPA）on puberty male reproductive system,40 puberty mice were randomly divided into 4 groups,including a control group and 3 BPA treatment groups（10,50 and 100 mg/kg,respectively）.Mice were injected intraperitoneally with BPA once per day for 7 days consecutiv ely while mice in the control group were immunized with equal volume of olive oi l.Testis and epididymis were weighed,organ coefficients were calculated a nd histopathological changes were examined.The contents of testosterone and lute inizing hormone in serum were detected by ELISA.In results,there was significant diffe-rence compared with the BPA groups i n the organ weights and the organ coefficients of testis and epididymis（P〈0.05,P〈0.01）.While germ cells in the BPA-treated grou ps were detached from basement membrance of semini-ferous tubules and serto li cells,the spermatogenic cells array loosely and disorderly.The level of testosterone and luteinizing hormone in the 50 and 100 mg/kg group s were significantly lower than those in the control group（P〈0. 01）.These results indicated that BPA could damage the development of reproductive sy stem of male mice.