中缅边境间日疟原虫环子孢子蛋白基因分型

Genotyping of Plasmodium vivax in China-Myanmar Border by Circumsporozoite Protein

根据间日疟原虫环子孢子蛋白(CSP)基因设计特异分型引物,利用巢式PCR技术(Nested-PCR)对采自中缅边境的间日疟患者血样作分型鉴定。检出的174份血样中,PV-I型热带族占54.6%(95/174)、温带族占35.6%(62/174)、PV-II型占2.9%(5/174)、混和感染占6.9%(12/174)。表明中缅边境的间日疟原虫存在4种基因型,以热带族为优势虫株,缅甸拉咱与云南腾冲间日疟原虫CSP基因型构成无差异(χ2=3.381,P>0.05)。

By using a series of type-specific primers for Plasmodium vivax circumsporozoite protein（CSP）gene and nested PCR,genotyping was conducted for the specimens of Plasmodium vivax isolated from China-Myanmar border.In 174 isolates of P.vivax,four genotypes,namely,tropical zone family strain,temperate zone family strain,genotype-mixed infection and PV-II type,were identified each accounting for 54.6 %,35.6%,6.9%,and 2.9%,respectively.The tropical zone family strain was dominant in the border area.There was no significant difference on the P.v CSP genotype constitution between Laza isolate of Myanmar and Tengchong isolate of Yunnan,China