曼氏迭宫绦虫裂头蚴超微结构的观察

Observation on the Ultrastructure of Spirometra mansoni Plerocercoid

取黑斑蛙肌肉内的曼氏迭宫绦虫裂头蚴,分别切取新鲜头颈部片段5～7个,经漂洗、固定、脱水、浸透、包埋、切片及染色处理后,透射电镜观察裂头蚴的超微结构。可见裂头蚴的体壁由皮层和实质区组成。微毛位于皮层的外表面,呈尖棘状。基质区内有许多形态各异的颗粒状分泌小体、囊泡、内质网和线粒体,其中线粒体多位于近基膜处。实质区有表层肌、皮层细胞、实质细胞、成石灰小体细胞和排泄系统等分布,并可见皮层细胞形成的胞质连接小管通过基膜伸向基质区。

Plerocercoids of Spirometra mansoni were collected from muscles of the frogs.Specimens were treated following the routine procedure,embedded,sliced and stained.The ultrastructure of plerocercoid was observed with trans-mission electron microscopy.It was found that the wall of plerocercoid consisted of tegument and parenchyma.Thornshape microtriches distributed over the outer surface of the tegument.Matrix zone had a lot of granular discoidal bodies,ves-icles,mitochondria and endoplasmic reticulum.Most of the mitochondria were near the basal membrane.Parenchyma zone consisted of muscular layer,tegument cells,parenchymal cells,excretory system,and so on.Many cytoplasmic pathways of tegumentary cells strech into muscular layer,suggesting that the tegument may be the absorptive site of nutrients.