哀牢山两类山地森林林冠及林下腐殖质微生物群落比较

Microbial community and its activities in canopy-and understory humus of two montane forest types in Ailao Mountains,Northwest China.

中山湿性常绿阔叶林、山顶苔藓矮林是云南哀牢山高海拔山地主要的原生森林植被类型.本文对哀牢山徐家坝地区两类森林林冠及各自林下地表腐殖质的理化特性、微生物组成、数量、生化活性、代谢活性及其季节动态,以及两类森林不同优势树种林冠腐殖质微生物数量及代谢活性进行了研究.结果表明:两类森林林冠腐殖质的真菌、放线菌的数量、微生物生物量C、N、固氮作用和纤维素分解作用的强度均显著高于林下地表腐殖质;中山湿性常绿阔叶林林冠腐殖质的纤维素分解菌、纤维素分解强度、微生物生物量C、N、有机碳、土壤呼吸强度等指标均显著高于苔藓矮林.在季节变化方面,湿季林冠腐殖质中真菌、细菌及好氧性固氮菌的数量以及呼吸强度均高于干季,放线菌则相反,纤维素分解菌干湿季间差异不显著.两类森林林冠腐殖质微生物数量及生化活性的季节变化幅度均大于林下地表腐殖质.不同树种之间冠层腐殖质的微生物数量差异显著,其中湿季的差异比干季更为明显.林冠层腐殖质的微生物在维系林冠附生植物多样性以及为林冠繁茂的附生植物生长发育提供所需的养分等方面具有重要的生态功能.

Mid-montane moist evergreen broadleaved forest（MMF） and top-montane dwarf mossy forest（DMF） are the two major natural forest types in subtropical mountainous area of Ailao Mountains,Northwest China.In this paper,a comparative study was made on the microbial composition,quantity,biochemical activity,metabolic activity,and their seasonal dynamics in the canopy-and understory humus of the two forest types.The composition,quantity,and metabolic activity of the microbes in the canopy humus of dominant tree species in MMF and DMF were also analyzed.In the canopy humus of the two forest types,the amounts of fungi and actinomycetes,microbial biomass C and N,and intensities of nitrogen fixation and cellulose decomposition were significantly higher than those in understory humus.Meanwhile,the amount of cellulose-decomposing microbes（ACDM）,cellulose decomposition intensity,microbial biomass C and N,and metabolic activity in the canopy humus of MMF were significantly higher than those of DMF.The amounts of bacteria,fungi,and aerobic nitrogen-fixing bacteria（ANFB） and the metabolic activity in the canopy humus of MMF and DMF were significantly higher in wet season than in dry season,while a contradictory trend was observed on the amount of actinomycetes.No significant difference was observed on the amount of ACDM between wet season and dry season.For the two forest types,the amounts of microbes and their biochemical activities in canopy humus had a larger seasonal variation range than those in understory humus.There was a significant difference in the amounts of the microbes in canopy humus among the dominant tree species in MMF and DMF,especially in wet season.The microbes in canopy humus played important roles in maintaining the biodiversity of epiphytes in the canopy,and in supplying the needed nutrients for the vigorous growth of the epiphytes.