摘要
目的:探讨尾加压素Ⅱ(U-Ⅱ)对肺腺癌A549细胞凋亡的影响及相关机制。方法:将体外培养A549细胞分为正常对照组、顺铂组(25μg/mL)及U-Ⅱ+顺铂组(10-7mol/L U-Ⅱ+25μg/mL顺铂),TUNEL法测定A549细胞凋亡,免疫细胞化学方法检测A549细胞中Bcl-2和Bax的表达。结果:与正常对照组相比,顺铂组肺腺癌A549细胞的凋亡率明显增加,Bcl-2蛋白表达明显降低,Bax表达明显升高(P<0.01);与顺铂组相比,U-Ⅱ则能够抑制A549细胞的凋亡,上调Bcl-2蛋白表达,下调Bax蛋白表达(P<0.01)。结论:U-Ⅱ可通过上调Bcl-2蛋白表达,下调Bax蛋白表达从而抑制肺腺癌A549细胞凋亡。
AIM:To explore the effect of Urotensin II on the apoptosis of human lung adenocarcinoma A549 cells and its underlying mechnism. METHODS: A549 cells were divided into three groups: Control group, Cisplatin group (25 μg/mL)and Urotensin II+Cisplatin group(10-7 mol/L U-Ⅱ+ 25 μg/mL cisplatin). The effect of Urotensin II on the apoptosis of A549 cells was determined by TUNEL method. The expressions of Bcl-2 and Bax were detected by immunocytochemistry. RESULTS:Comparing with Control group, the apoptotic rate and the expression of Bax were increased, while the expression of Bcl-2 was decreased in Cisplatin group(P0.01). Urotensin II significantly inhibited Cisplatin-induced apoptosis of A549 cells and antagonized Cisplatin-induced down-regulation of Bcl-2 and up-regulation of Bax(P0.01). CONCLUSION: Urotensin II inhibits the apoptosis of human lung adenocarcinoma A549 cells by up-regulating the expression of Bcl-2 and down-regulating the expression of Bax.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2010年第7期742-746,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
江苏省高校自然科学基金(08KJD310010)
徐州医学院院长专项人才基金(08KJZ05)
徐州医学院肿瘤生物治疗重点实验室开放课题基金(C0804)资助