主成分分析法对促透剂促透效果的评价

Evaluation of promoting effects of transdermal enhancers with principle components analysis method

目的:运用主成分分析法综合评价几种促透剂的促透效果。方法:以5-氟尿嘧啶为模型药物,利用离体透皮吸收装置,研究丁香油、广藿香油、荆芥油、氮酮、薄荷醇、油酸、樟脑促透剂在家兔背部皮肤上的透皮效果,计算累积透过量、透皮速率、增渗倍数和滞后时间,并运用主成分分析法评价促透效果。结果:油酸、丁香油、薄荷醇、广藿香油、氮酮、荆芥油都有促透作用,作用依次递进,2%荆芥油最强;氮酮、丁香油、樟脑、广藿香油、油酸、薄荷醇、荆芥油都有储库效应,效应依次递进。结论:主成分分析法可客观地综合评价促透剂的促透效果。

AIM：To evaluate the promoting effects of several transdermal enhancers with principle components analysis method. METHODS： Using 5-fluorouracil（5-Fu）as model drug,the promoting effects of caryophyllus oil, agastache rugosus oil, chaste oil, azone, menthol crystal, oleic acid and camphor were detected by transdermal absorption experimentation in rabbit skin in vitro, indexes such as cumulative permeation quantity, penetrating rates, enhancement ratio and lag time were calculated, finally the principle components analysis method was used to evaluate the promoting effects of those transdermal enhancers. RESULTS：Oleic acid, caryopyllus oil, menthol crystal, agastache rugosus oil, azone and chaste oil all promoted 5-Fu through the skin, and the effects of these transdermal enhancers increased in sequence,2% chaste oil occupied the strongest position. Azone, caryopyllus oil, camphor, agastache rugosus oil, oleic acid, menthol crystal and chaste oil all showed the deposit effect, and those enhanced in sequence, too. CONCLUSION： The method of principle components analysis could evaluate the promoting effects of transdermal enhancers objectively and synthesizly.