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吉西他滨联合X射线照射对肺癌细胞系放射增敏作用的初步研究 被引量:4

Radiosensitizing effect of gemcitabine on human lung cancer cells : A preliminary in vitro study
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摘要 目的 探讨吉西他滨(GEM)是否对非小细胞肺癌具有放射增敏作用,并对GEM的放射增敏机制进行初步探讨.方法 用克隆形成分析法观察GEM对p53基因突变的人肺腺癌细胞系(973细胞)的放射增敏效应.流式细胞术观察照射前后973细胞周期分布和细胞凋亡,分析其与p53基因突变是否为放射增敏机制.结果 10 nmol/L GEM照前、照后给药均具有极轻微放射增敏作用;100 nmol/L GEM照前、照后给药时均具有明显放射增敏作用,且照前给药组的增敏作用明显强于照后给药组.p53基因突变影响细胞周期再分布及细胞凋亡,但与GEM的放射增敏作用无关.结论 100 nmol/L GEM具有明显放射增敏作用,p53基因突变、细胞周期再分布及细胞凋亡不是GEM放射增敏作用的主要机制. Objective To investigate whether gemcitabine (GEM) could enhance radiosensitivity of human non-small cell lung cancer cells and its related mechanism.Methods Clonogenic assay was used to analyze radiosensitivity enhancement by GEM on p53 mutant human lung adenocarcinoma cell line 973.Alterations of cell cycle distribution and apoptosis were measured by flow cytometry.Results Mild radiosesitizing effect was observed when 10 nmol/L GEM was administrated before or after irradiation.Marked radiosesitizing effect was demonstrated when 100 nmol/L GEM was administrated before or after irradiation, with much stronger effect of pre-irradiation GEM treatment.Mutation of p53 gene affected cell cycle redistribution and cell apoptosis, but had no relationship with radiosensitivity enhancement of GEM.Conclusions 100 nmol/L GEM could significantly enhance radiosensitivity of human lung cancer cells.However, this effect may not be associated with p53 gene mutation, cell cycle redistribution or cell apoptosis.
出处 《中华放射肿瘤学杂志》 CSCD 北大核心 2010年第5期471-474,共4页 Chinese Journal of Radiation Oncology
关键词 细胞系 肺肿瘤 吉西他滨 X射线照射 克隆分析 流式细胞术 放射增敏 Cell line,lung neoplasms Gemcitabine X-ray irradiation Clonogenic assay Flow cytometry Radiosensitivity
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参考文献19

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