蛹虫草退化菌株的特征研究

Characteristics of the degenerate strains of Cordyceps militaris

蛹虫草作为虫草资源和健康食品之一业已产业化人工栽培。但是,蛹虫草菌种在继代培养过程中的频繁退化不仅导致其产量的急剧下降,而且成为影响行业发展的核心技术问题。作者研究了蛹虫草退化菌株与正常菌株在交配型、dsRNA感染、色度和脱氢酶活性的特征差异性。结果表明,退化菌株的脱氢酶活性和在色度培养基中的脱色能力都明显弱于正常菌株;而在交配型和dsRNA感染方面,两者未表现出差异。这一结果为蛹虫草人工栽培过程中退化菌种的早期检测提供了快速的方法。

Cordyceps militaris has been commercially cultivated. However, the degenerate subcultures of C. militaris have increasingly reduced the production of this economically important fungus. The characteristics of dehydrogenase activity, decolorization activity, mating-type and dsRNA infection of the degenerate strains were analyzed for comparison with the wild strains of C. militaris. The results showed that the degenerate strains had significantly lower dehydrogenase activity and decolorization activity, while no differences in characteristics of mating-type and dsRNA infection were observed between degenerated strains and normal strains. The result provides a quick method to detect early degeneration of C. militaris inocula during the process of artificial cultivation.