Tspan-1在结直肠肿瘤组织中的表达及其意义

Expression of Tspan-1 in benign and malignant lesions of the colon

目的 研究Tspan-1基因在结直肠癌组织中的表达,分析其与临床病理因素及预后的关系.方法 采用免疫组化ELIVISION二步法,检测Tspan-1基因蛋白在80例结直肠癌组织,13例结肠腺瘤组织及27例正常结肠黏膜组织中的表达.结果 本组80例结肠癌组织中Tspan-1阳性表达率为90%,13例结肠腺瘤组织中表达率为23%,27例正常结肠黏膜中表达率为7%,3组之间相比差异均具有统计学意义（P＜0.01）.Tspan-1阳性表达在低中分化癌中均显著高于高分化癌（P＜0.01）,有淋巴结转移组明显高于无淋巴结转移组（P＜0.01）,TNM分期Ⅲ～Ⅳ期组中表达显著高于Ⅰ期组（P＜0.01）.Tspan-1与肿瘤浸润深度、5年生存率有显著相关性（P＜0.01）,与性别、年龄、部位、肿瘤大小、病理类型等无关（P＞0.05）.结论 Tspan-1基因表达可以被用作判断结直肠癌预后的一个标志 Tspan-1蛋白的表达可能在大肠腺瘤的形成中起重要作用.

Objective To study the protein expression of Tspan-1 gene in colorectal carcinoma,and investigate the relationship between the expression and biologic behavior of colorectal carcinoma. Methods Immunohistochemical ELIVISION two-step method was used to detect the protein expression of Tspan-1 gene in 80 cases of colorectal carcinoma,13 cases of colorectal adenoma and 27 cases of normal colorectal tissues. Results The positive expression rate of Tspan-1 in colorectal carcinoma was significantly higher than that in colorectal adenoma and normal colorectal tissues,there was significant difference in the expression among the three groups.The positive expression rate of Tspan-1 was 90% in colorectal carcinoma,23% in colorectal adenoma and 7% in normal colorectal tissues （P 〈 0.01）.The positive expression rate of Tspan-1 in poor and moderately differentiated carcinoma was significantly higher than that in well-differentiated carcinoma （P 〈 0.01）.The expression of Tspan-1 was significantly associated with lymph node metastasis （P〈0.01）,and with the 5-year survival rate of colorectal carcinoma patients,with also TNM stage and the depth of tumor invasion （P 〈0.01）,and while it was not associated with gender,age,location,tumor size,and type （P 〉 0.05）. Conclusion The expression of Tspan-1 gene could be as a marker predicting the prodnosis of colorectal adenocarcinoma patients,also it may play an important role in the pathogenesis of colorectal adenoma.