Ni^(2+)与EGCG相互作用对人牙龈成纤维细胞的生物学效应

The biological effect of interaction of Ni^(2+) and epigallocatechin gallate on human gingival fibroblast cells

目的分析表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)、Ni2+及其相互作用对人牙龈成纤维细胞(human gingival fibroblast cells,HGF)的生物学效应。方法用MTT法检测HGF的存活率,运用彗星电泳检测细胞的DNA损伤情况,使用流式细胞仪法检测HGF周期变化。结果 Ni2+可呈时间依赖性抑制HGF的生长,造成细胞DNA损伤,并且可使细胞周期阻滞于S期,诱导细胞凋亡。高浓度的EGCG(>200μmol/L)可明显抑制HGF生长,使细胞的DNA损伤。Ni2+与EGCG相互作用后增加了对HGF的抑制(P<0.01),DNA的损伤及凋亡细胞也明显增加(P<0.05)。结论 Ni2+和EGCG相互作用后使Ni2+对HGF的抑制作用增强,加剧HGF的DNA损伤和细胞周期阻滞,并明显增加细胞凋亡。

Objective To determine the biological effects of epigallocatechin gallate（EGCG）,Ni2＋ and their interactions on human gingival fibroblast（HGF） cells. Methods The viability of cells was determined by the MTT reduction assay.To measure DNA damage,we followed a modified version of comet assay.Cell cycle was analyzed by flow cytometry. Results Ni2＋ significantly inhibited the growth of HGF cells.Concentrations of Ni2＋ greater than 100 μmol/L significantly increased DNA strand breaks damage and arrested the cell cycle.The high concentrations of EGCG（greater than 200 μmol/L） could inhibit the growth of HGF cells.Greater than 150 μmol/L EGCG could increase the DNA strand break damage.The co-treatment of Ni2＋ and EGCG could increase Ni2＋-induced inhibit effect on HGF cells（P0.01）,and the DNA damage was significantly increased（P0.01）.Conclusions Ni2＋ mixed with EGCG could increase the Ni2＋-induced damage on the HGF cells and the blockage of cell cycle.