喀什维吾尔族妇女宫颈癌与HLA-DRB1*15和HLA-DRB1*04的关系

The relationships between HLA-DRB1*15 or HLA-DRB1*04 and cervical cancer in Kashgar Uyghur women

目的探讨人类白细胞抗原HLA-DRB1等位基因多态性与新疆宫颈癌高发区喀什维吾尔族妇女宫颈癌的关系。方法采用聚合酶联反应序列特异性寡核苷酸探针(polymerase chain reaction sequence-specificoligonucleotide,PCR-SSO)法检测90例新疆喀什维吾尔族宫颈癌患者及90例当地正常妇女宫颈组织中HLA-DRB1的等位基因。结果等位基因HLA-DRB1*15在宫颈癌组中出现的频率明显高于对照组,差异有统计学意义(χ2=4.05,P<0.05,OR=2.002),表明HLA-DRB1*15可能是喀什维吾尔族妇女宫颈癌的易感基因;而等位基因HLA-DRB1*04在宫颈癌组中出现的频率明显低于对照组,差异有统计学意义(χ2=7.283,P<0.05,OR=0.432),表明HLA-DRB1*04可能是喀什维吾尔族妇女宫颈癌的保护基因;HLA-DRB1的其他等位基因在两组中出现的频率差异无统计学意义(P>0.05)。结论 HLA-DRB1*15可能与新疆宫颈癌高发区喀什维吾尔族妇女对宫颈癌的遗传易感性有关,是当地维吾尔族妇女宫颈癌的易感基因;而HLA-DRB1*04可能是喀什维吾尔族妇女宫颈癌的保护基因。两组基因的检测在维族妇女高危人群的筛选及群体遗传干预工作中有重要意义。

Objective To investigate the relationships between the HLA-DRB1 allele polymorphisms and the Uyghur women＇s susceptibility to cervical carcinomas. Methods The HLA-DRB1 alleles were detected in 90 cervical cancer patients and 90 cases of control whom all from Kashgar by using the polymerase chain reaction sequence-specific oligonucleotide（PCR-SSO） method. Results The frequency of HLA-DRB1＊15 in cervical cancer was significantly higher than that in the control group（χ2=4.05,P0.05,OR=2.002）,while the frequency of HLA-DRB1＊04 was significantly lower than that in the control group（χ2=4.05,P0.05,OR=2.002）.The frequencies of other HLA-DRB1 alleles did not show statistical differences between the two groups. Conclusions HLA-DRB1＊15 allele may be the susceptible gene to cervical cancer in the Uyghur women from Kashgar,while HLA-DRB1＊04 allele may be the protective gene.Detecting of the two genes in high risk population may play an important role in screening and interferencing researches of cervical cancer in Uyghur women.