摘要
目的 观察氟对大鼠骨组织Runx2 mRNA和蛋白表达水平的影响.方法 14只SD大鼠按体质量随机分为对照组[饮用自来水,含氟（F-）＜0.06 mg/L]、染氟组（饮水含F-50 mg/L）,饲养4个月后股动脉放血处死.采用蛋白质印迹法（Western blot）检测Runx2蛋白表达水平;采用RT-PCR法检测Runx2 mRNA的表达水平.结果 与对照组相比,染氟组大鼠骨组织Runx2 mRNA及蛋白表达水平（2.287±0.261、0.929±0.229）均高于对照组（0.995±0.123、0.317±0.068,t值分别为11.85、6.78,P均＜0.05）.结论 氟可导致大鼠骨组织Runx2 mRNA及蛋白表达水平增高,Runx2可能参与了氟引起的骨骼损伤的发生机制.
Objective To investigate the effect of fluoride on expression of Runx2 mRNA and protein in bone tissue of rats. Methods Fourteen SD rats were randomly divided into two groups： control group（tap water with fluoride 〈 0.06 mg/L）, and fluorosis group（fluoride 50 mg/L in water）. After 4 moths, expressions of both mRNA and protein of Runx2 in rat bone tissue were determined by RT-PCR and Western blotting. Results The results showed that the expression of Runx2 mRNA and protein in fluoride-treated bone tissue were 2.287 ± 0.261 and 0.929 ± 0.229, respectively, both of which were significantly higher than those of control group（0.995 ± 0.123,0.317 ± 0.068, t = 11.85,6.78, P 〈 0.05）. Conclusions Fluoride can increase the expression of Runx2 mRNA and protein in bone tissue of rats, and Runx2 may be involved in the pathogenesis of bone injury caused by fluoride.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2010年第5期493-495,共3页
Chinese Jouranl of Endemiology
基金
贵州省科技厅国际合作重点项目[黔科合外G字（2007）400119]、贵州省科技厅社会发展攻关项目[黔科合SY字（2008）3054]
贵州省贵阳市科技局基金资助项目（T2009-3）
科技部国际合作项目（2010DFB30530）
