四妙勇安汤对实验性兔动脉粥样硬化易损斑块的影响

Effects of"Simiao Yongan Decoction"on extracellular matrix in rabbit's atherosclerosis vulnerable plaque

目的探讨四妙勇安汤对AS易损斑块的影响及机制。方法随机将40只日本大耳白兔分为正常组10只,实验组30只。正常组予普通饲料,实验组利用复合因素制备兔AS易损斑块模型,8周时实验组随机分为模型组、辛伐组、四妙组,24周取材。检测动脉壁内膜厚度(IT)、内中膜厚度比(IT/MT)、斑块纤维帽厚度(FCT)、纤维帽与内中膜厚度比(FCT/IMT)、胶原含量(CA)、α-平滑肌肌动蛋白(α-SMA)、基质金属蛋白酶-9(MMP-9)、肿瘤坏死因子-α(TNF-α)及核因子-κB(NF-κB)阳性面积百分比。结果模型组各指标与正常组比较,差异均有统计学意义(P<0.05,P<0.01)。四妙组IT、IT/MT均低于模型组(P<0.05),FCT和FCT/IMT均高于模型组(P<0.01);斑块内CA阳性面积百分比均高于模型组(P<0.01),α-SMA阳性面积百分比低于正常组MMP-9和NF-κB阳性面积百分比均低于模型组(P<0.01,P<0.05)。结论四妙勇安汤能够稳定AS易损斑块,其作用机制可能是通过抑制MMP-9、TNF-α及NF-κB表达实现的。

Objective To observe the intervening effect and acting mechanism of ＂Simiao Yongan Decoction＂ on extracellular matrix in rabbit＇ s atherosclerosis （AS） vulnerable plaque. Methods Forty Japanese white rabbits were randondy assigned into two groups： 10 rabbits in the normal group, and 30 rabbits in experiment group which was established to be AS vulnerable plaque model by high-fat diet combined with immune injury and femoral arterial balloon tearing. On the 8th week, 30 rabbits in experiment group were randomly divided into three groups： model group, ＂SimiaoYongan Decoction＂ group and simvastatin group, 10 rabbits in each group. On the 2gth week, the intimer thickness（ IT）, ratio of intimer thickness to media thickness（ IT/MT）, fiber cat thickness（FCT）, FCT/IMT, collagen, α-SMA, tumor necrosis factor-α（ TNF-α）, matrix metalloproteinase-9 （MMP-9） and NF-κB expression in aortic wall were measured. Results Compared with the normal group, the IT, collagen, α-SMA and MMP-9 expression in aortic wall in the model group were higher （P 〈0.05） ; compared with the model group, in the ＂SimiaoYongan Decoction＂ group the IT, IT/MT, MMP-9 and NF-scB expression were lower（P 〈0.05, P 〈0.01 ）, and FCT, FCT/MT, collagen and α-SMA were higher（P 〈0.05, P 〈0.01 ）. Conclusion ＂SimiaoYongan Decoction＂ can stabilize atherosclerosis vulnerable plaque by inhibiting MMP-9, TNF-α and NF-κB expressions.