病毒性心肌炎小鼠辅助性T淋巴细胞17亚群的变化

Alteration of Th17 cells in mice with coxsackie virus induced myocarditis

目的 观察病毒性心肌炎小鼠不同时间点辅助性T淋巴细胞17（Th17）亚群的变化,探讨其在病毒性心肌炎发病中的作用.方法 用Balb/c小鼠建立柯萨奇病毒B3（CVB3）心肌炎小鼠模型（实验组）,对照组注射等量磷酸盐缓冲液,在注射的第0、7、14、21、28和42天苏木精-伊红染色观察心肌病理改变并计算心肌病理积分,流式细胞仪检测小鼠脾脏Th17细胞亚群的比例（Th17/CD4^＋）.结果 实验组7 d亚组小鼠心肌组织病理积分（1.8±0.5）高于0 d亚组,14 d亚组病理积分在实验组各亚组中最高（2.8±0.4）,从21 d亚组起病理积分开始低于14 d亚组,实验组各亚组与对照组相应时点亚组比较差异均有统计学意义（P均〈0.05）.实验组7 d亚组小鼠脾Th17/CD4^＋[（2.23±0.89）%]高于实验组0 d亚组,28 d亚组脾Th17/CD4^＋最高[（5.00±0.81）%],42 d亚组脾Th17/CD4^＋[（2.35±0.35）%]低于实验组28 d亚组,与对照组相应时点亚组比较差异均有统计学意义（P均〈0.05）.结论 病毒性心肌炎小鼠中存在Th17细胞,其可能参与了病毒性心肌炎的炎性过程.

Objective To observe the alteration of T helper cells 17 （Th17） in mice with acute viral myocarditis （VMC） induced by coxsackie virus B3 （CVB3）, explore the role of Th17 in mice VMC.Methods CVB3 or PBS was peritoneally injected to Balb/c male mice. Pathological scores were determined in hematoxylin-eosin stained sections and flow cytometric analysis was used to evaluate the frequencies of Th17 subsets in CD4^＋ T cells on 7, 14,21,28 and 42 days after virus injection. Results There were significant difference of the pathological scores between the VMC mice and the control ones （ P 〈 0. 05 ）. The pathological scores of 7 d VMC subgroup were higher（ 1.8 ± 0. 5） than those of O d VMC subgroup, and the scores of 14 d subgroup were highest （2. 8 ±0. 4） among the six subgroup of VMC mice, and then showed a decline tendency from 21 d group. Statistical difference of the proportion of Th17 cells were seen between the VMC and controls on different time points （ P 〈 0. 05 ）. When compared with the 0 d VMC subgroup the proportion of spleen Th17 cells increased in 7 d VMC subgroup [（2. 23 ±0. 89）%], and peaked on 28 d [（5.00 ±0.81）%]. The results of Th17 proportion were lower than those of the 28 d subgroup.Conclusions Our data show that differentiated Th17 cells might be involved in the inflammation process of CVB3 induced VMC in mice.