Ghrelin免疫反应阳性神经元在鸡脑中的定位分布、发育性变化及禁食对其影响

Localization,Distribution and Development of Ghrelin-immunopositive Neurons and Influence of Fasting on Them in Brain of Broiler Chickens

本试验应用免疫组织化学方法和显微图像分析技术,研究了鸡脑Ghrelin免疫反应阳性神经元的定位分布,分析了2、16、30、44、58日龄鸡下丘脑Ghrelin免疫反应阳性神经元的发育性变化规律,并探讨了30日龄鸡分别禁食12、24、48h及重新采食4h对下丘脑Ghrelin免疫反应阳性神经元的影响。结果表明,在下丘脑弓状核、室旁核、内侧核、室周区、内侧区、外侧区,丘脑卵圆核、圆核,中脑丘中央灰质层、红核,脑桥前庭腹外侧核,小脑内侧核、小脑皮质颗粒层,大脑皮质多形细胞层等,均可观察到Ghrelin免疫反应阳性神经元;随着日龄的增长,下丘脑主要核团中的Ghrelin免疫反应阳性神经元的数量增多,免疫反应强度增强,但细胞密度下降;在禁食条件下,下丘脑Ghrelin免疫反应阳性神经元的免疫反应强度减弱,细胞密度下降。

In this paper,the localization and distribution of ghrelin-immunopositive neurons in the brain,the development of these neurons in the major nucleus of hypothalamus in 2,16,30,44 and 58-day-old,and the influence of fasting 12,24,48hand refeeding 4hin 30-day-old on these neurons in broiler chickens were investigated by immunohistochemical staining and microscopic image analysis.The results showed that the ghrelin-immunopositive neurons were observed in the nucleus periventvicularis accuatas,the nucleus paramedianus,the nucleus medialis,the area periventricularis,the area rostromedialis and the area rotrolateralis hypothalami,the nucleus rotundus and the nucleus ovoidali,the gray matter of colliculus mesencephali and the nucleus ruber,the nucleus vestibularis ventrolateralis,the nucleus cerebellaris medialis and the granular layer of cerebellar cortex,and the polymorphic layer of cerebral cortex;with the chicken growth, the number of these neurons was increased,their immunopositive reaction level was enhanced, but their density was decreased;during the fasting and refeeding,their immunopositive reaction level was weakened and their density was decreased.