水溶性苜蓿多糖对肉仔鸡T、B淋巴细胞体外增殖的影响

Effects of Water Soluble Alfalfa Polysaccharides on Cell Proliferation of Broiler Lymphocytes in Vitro

为探讨水溶性苜蓿多糖(WSAP)对体外培养的肉仔鸡淋巴细胞增殖的影响。本研究将不同浓度的WSAP分别加入到体外培养的用伴刀豆球蛋白A(ConA)刺激的肉仔鸡外周血、胸腺、脾脏淋巴细胞中和用细菌脂多糖(LPS)刺激的肉仔鸡外周血、脾脏、法氏囊淋巴细胞中,培养48h后用四甲基偶氮唑蓝(MTT)比色法测定淋巴细胞增殖的变化。结果显示:(1)在本试验中,当WSAP在一定浓度范围(4～20μg·mL-1)内时,T、B淋巴细胞的OD值比对照组高,且随着多糖浓度的增加,数值先增高(1～20μg·mL-1)后降低(100～200μg·mL-1);(2)同浓度WSAP对肉仔鸡外周血及不同的免疫器官的刺激强度不同,相同日龄时,肉仔鸡外周血和脾脏T、B淋巴细胞的OD值分别小于胸腺和法氏囊的OD值;(3)WSAP对相同日龄的肉仔鸡外周血及不同的免疫器官的最佳刺激浓度不同,对外周血的最佳刺激浓度为20μg·mL-1,脾脏为8μg·mL-1,法氏囊和胸腺为15μg·mL-1。本试验中WSAP对肉仔鸡体外淋巴细胞增殖的刺激浓度在8、15、20μg·mL-1时效果较好,这些结果表明一定浓度的WSAP可以促进肉仔鸡淋巴细胞的增殖,且存在一定的量效、时效关系和组织特异性。

To investigate the effects of Water Soluble Alfalfa Polysaccharides（WSAP）on cell proliferation of broiler lymphocytes in vitro.Different concentrations of WSAP were added into cultured with ConA stimulated chicken peripheral blood,thymus,spleen lymphocytes and into cultured with LPS stimulated chicken peripheral blood,spleen,bursa of fabricius,cultured for 48 hours,use MTT colorimetric determination of lymphocyte proliferation.The results showed that （1）In this experiment,the OD values of T,B lymphocytes in a certain concentration of WSAP （4-20μg·mL^-1）were higher than those in control groups,with the polysaccharide concentration increased,OD values increased first（1-20μg·mL^-1）and then decreased（100-200μg·mL^-1）; （2）The same concentration WSAP on broiler peripheral blood and other immune organs,the stimulus intensity were different,peripheral blood and spleen T,B lymphocytes OD values were less than the thymus and bursa lymphocytes OD values;（3）The best stimulus concentration for peripheral blood and other organs were difference,for peripheral blood was 20μg·mL^-1,for spleen was 8μg·mL-1,for bursa and thymus with 15μg·mL^-1.In this experiment,the concentration of WSAP with 8,15,20μg·mL^-1 could promote the broiler lymphocyte proliferation, which may be the one of the mechanisms that polysaccharides could enhance immune function.