白藜芦醇对体外高糖刺激下小鼠脑微血管内皮细胞解偶联蛋白基因表达的影响

Effects of resveratrol on uncoupling protein mRNA expression in brain microvessel endothelial cells under hyperglycemic condition in vitro

目的观察高糖对小鼠脑微血管内皮细胞解偶联蛋白(uncoupling protein,UCP)基因表达的影响,并探讨白藜芦醇的调节作用。方法通过体外培养小鼠脑微血管内皮细胞系bEnd.3,采用RT-PCR方法观察不同浓度葡萄糖(5.5,25mmol/L)刺激及白藜芦醇(25μmol/L)干预时UCP基因表达的变化。结果 25mmol/L葡萄糖糖刺激24h能上调UCP4、UCP5 mRNA表达(P<0.05),而对UCP1、UCP2基因表达无明显影响;白藜芦醇能上调基础状态及高糖状态下的UCP5基因表达(P<0.05),能抑制高糖引起的氧自由基增加(P<0.05)。结论高糖刺激可特异性上调小鼠脑微血管内皮细胞UCP4、UCP5基因表达;白藜芦醇能降低高糖诱导的氧自由基生成,同时上调细胞UCP5基因的表达。

Objective To study the effect of hyperglycemia on uncoupling protein （UCP） mRNA expression in mouse brain microvessel endothelial cell line bEnd.3, and to investigate the regulatory effect of resveratrol on the expression.Methods Mouse bEnd.3 cells were cultured in vitro.The mRNA levels of UCP1-5 in bEnd.3 cells were determined by RT-PCR after exposure to different concentrations of glucose（5.5、25 mmol/L） and resveratrol （25 μmol/L）.Results We found that exposure to 25 mmol/L glucose for 24 h up-regulated the expression of UCP4 and UCP5 mRNA levels （P〈0.05）, but had no noticeable influence on the expression of UCP1 and UCP2 mRNA levels. Resveratrol increased UCP5 mRNA levels under baseline and hyperglycemia conditions （P〈0.05）, and it decreased reactive oxygen specices（ROS） induced by hyperglycemia condition （P〈0.05）.Conclusion High glucose stimulation can specifically up-regulate UCP4 and UCP5 mRNA expression in mouse brain microvessel endothelial cells. Resveratrol can greatly decrease ROS production induced by hyperglycemia condition and up-regulate UCP5 mRNA expression.