骨形态发生蛋白2基因转染软骨细胞注入腰椎间盘损伤模型:10周测量蛋白多糖和胶原含量

Injection of chondrocyte transfected by bone morphogenetic protein-2 into intervertebral disc degeneration models:Contents of proteoglycan and collagen at 10 weeks

背景:研究表明骨形态发生蛋白2基因转染软骨细胞可促进体外培养的髓核细胞合成细胞外基质,但其对体内退变椎间盘组织代谢的影响却罕见报道。目的:探讨骨形态发生蛋白2基因转染细胞移植治疗椎间盘退变的可行性。方法:用新西兰大白兔建立腰椎间盘损伤动物模型,建模后随机分为细胞移植组和对照组。细胞移植组注入骨形态发生蛋白2基因转染的软骨细胞,对照组注入等剂量生理盐水,两组动物在注射后2,4,6,8和10周分别取出退变的椎间盘髓核组织,并测量其蛋白多糖及胶原含量。结果与结论:与对照组比较,细胞移植组髓核蛋白多糖及胶原含量均有明显增高(P<0.05)。结果提示椎间盘退变的标志之一可能为髓核内细胞外基质含量的减少,而骨形态发生蛋白2基因转染细胞移植的方法可促进细胞外基质合成,改善退变髓核的生物活性,延缓椎间盘的退变。

BACKGROUND：Previous studies demonstrated that bone morphogenetic protein-2（BMP-2） transfected chondrocytes can promote nucleus pulposus cells synthesize extracellular matrix,but its effect on metabolism of degenerating intervertebral disc remains poorly understood.OBJECTIVE：To study the feasibility of the application of BMP-2-transfected chondrocytes on intervertebral disc degeneration.METHODS：New Zealand rabbits were prepared for intervertebral disc degeneration models.The model success was randomly divided into cell transplantation and control groups,which received injection of BMP-2-transfected chondrocyte or physiological saline,respectively.The intervertebral disc tissues were harvested at 2,4,6,8 and 10 weeks after injection,and the proteoglycan and collagen contents were measured.RESULTS AND CONCLUSION：Compared with the control group,the proteoglycan and collagen contents of the cell transplantation group were obviously increased（P 0.05）.The findings revealed that,extracellular matrix reduce was one important indication of intervertebral disc degeneration.Transplantation of BMP-2-transfected chondrocytes can raise the content of extracellular matrix,improve biological activity of degenerative nucleus pulposus,and delay intervertebral disc degeneration.