兔椎间盘成骨作用中的转基因人骨形态发生蛋白7

Osteogensis effect of transgenic human bone morphogenetic proteins-7 on rabbit intervertebral disc

背景:对于骨形态发生蛋白的生物活性分析中发现,极其微量的骨形态发生蛋白在成年个体中被分泌和储存在骨基质和成骨、成软骨等细胞中,当骨折时这些骨形态发生蛋白在骨折区域释放,诱导周围的间充质细胞发生化学趋化、聚集、分化并形成软骨,从而最终形成成熟的骨结构。目的:验证转基因人骨形态发生蛋白7在兔椎间盘组织中的成骨作用。方法:健康成年新西兰大白兔随机分成4组。分别于不同的腰椎间盘注入骨形态发生蛋白7溶液,生理盐水,或使用金属手术器械行腰椎间盘单纯破坏,并设立未干预的腰椎间盘作为空白对照。术后第6周应用组织学观察注射区内新骨的形成情况。结果与结论:各组动物注射生理盐水的椎间盘和空白椎间盘均为阴性结果。注射骨形态发生蛋白组的新生骨的面积显著大于单纯破坏组(P<0.05)。注射骨形态发生蛋白组新生骨面积组内差异在放大40倍和100倍时,差异无显著性意义(P>0.05)。结果证实转基因人骨形态发生蛋白7能将家兔的椎间盘转化为新生软骨组织。

BACKGROUND：Small amount of bone morphogenetic protein（BMP） are secreted and stored in bone matrix,osteoblast and chondroblast of adult body.After bone fracture,these BMP are released at the fracture area and induce surrounding mesenchymal cells to chemotaxis,aggregate,differentiate,and form cartilage,thereby,form mature bone structure ultimately.OBJECTIVE：To evaluate osteogensis effect of transgenic human bone morphogenetic proteins-7（rhBMP-7） in rabbit intervertebral disc.METHODS：Healthy,adult,New Zealand,white rabbits were randomly divided into four groups,which were received injection of rhBMP-7,sodium chloride or simple damaged intervertebral discs.Simultaneously,normal intervertebral discs were served as controls.At 6 weeks,the intervertebral discs of rabbits were examined by histological detection to observe the new bone formation in injected area.RESULTS AND CONCLUSION：Negative results could be found in the sodium chloride and control groups.The new bone area of the rhBMP-7 group was greater than that of the simple damaged group（P 0.05）.There were no significant between rhBMP-7 group and the simply damaged groups when the differences were magnified for 40-and 100-fold（P 0.05）.Transgenic rhBMP-7 can change rabbit intervertebral disc to newborn cartilage.